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ApaI
ApaI restriction site:
G GGCC | C
C | CCGG G
Supplied buffer: L
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Acetobacter pasteurianus sub. pasteurianus
- Concentration: 8–20 units/μl; 30–60 units/μl (high concentration)
- Substrate for unit definition: lambda DNA
- Genome DNA analysis: Staphylococcus aureus genome DNA
- Definition of activity: 3 units for lambda DNA as substrate is equivalent to 1 unit for an Ad2 substrate
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
ApaI restriction site:
G GGCC | C
C | CCGG G
Supplied buffer: L
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Acetobacter pasteurianus sub. pasteurianus
- Concentration: 8–20 units/μl; 30–60 units/μl (high concentration)
- Substrate for unit definition: lambda DNA
- Genome DNA analysis: Staphylococcus aureus genome DNA
- Definition of activity: 3 units for lambda DNA as substrate is equivalent to 1 unit for an Ad2 substrate
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
