Annexin V Apoptosis Assay
Annexin V Apoptosis Assay
Brand: Takara Bio.
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Annexin V Apoptosis Assay
The ApoAlert Annexin V-FITC Apoptosis Kit provides a simple and effective method to detect one of the earliest events in apoptosis—the externalization of phosphatidylserine (PS)—in living cells. Soon after apoptosis is induced, PS is translocated from the inner leaflet of the plasma membrane to the outer leaflet. This assay uses annexin V, which has a strong and specific affinity for PS, to monitor the PS translocation that occurs due to apoptosis. Labeled annexin V provides a simple staining assay to monitor apoptosis by flow cytometry or fluorescence microscopy.
Overview
- Easy to use: non-enzymatic assay, no fixation
- Simple protocol: one-step procedure that requires just 10 min to perform
- Versatile: suitable for both adherent and suspension cells
Applications
- Early apoptosis detection
- Monitor phosphatidylserine translocation by flow cytometry or fluorescence microscopy
Annexin V-FITC staining of apoptosis in Jurkat T cells
Annexin V-FITC staining of apoptosis in Jurkat T cells. Apoptosis was induced with 200 ng/ml anti-Fas (lgM,CH11). At the indicated times, cells were incubated for 5 minutes with 1.0 μg/ml Annexin V-FITC in binding buffer, and 5,000 cells were examined by BD FACS analysis. For details see Martin et al., 1995. Data reproduced with author’s permission.
Annexin V-FITC apoptosis: externalization of phosphatidylserine (PS) right after the induction of apoptosis
Annexin V-FITC apoptosis: externalization of phosphatidylserine (PS) right after the induction of apoptosis.
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Annexin V-FITC staining of apoptosis in Jurkat T cells
Annexin V-FITC staining of apoptosis in Jurkat T cells. Apoptosis was induced with 200 ng/ml anti-Fas (lgM,CH11). At the indicated times, cells were incubated for 5 minutes with 1.0 μg/ml Annexin V-FITC in binding buffer, and 5,000 cells were examined by BD FACS analysis. For details see Martin et al., 1995. Data reproduced with author’s permission.
Annexin V-FITC apoptosis: externalization of phosphatidylserine (PS) right after the induction of apoptosis
Annexin V-FITC apoptosis: externalization of phosphatidylserine (PS) right after the induction of apoptosis.
