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AfaI (RsaI)
AfaI (RsaI) restriction site:
GT | AC
CA | TG
Supplied buffer: T+BSA
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Acidiphilium facilis
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
AfaI (RsaI) restriction site:
GT | AC
CA | TG
Supplied buffer: T+BSA
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Acidiphilium facilis
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
