Advantage HF 2 PCR Kit
Advantage HF 2 PCR Kit
Brand: Takara Bio.
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Advantage HF 2 PCR Kit
Advantage HF 2 PCR Kit
The Advantage HF 2 PCR Kit is a high-performance PCR system optimized for error-free amplification of genomic and cDNA targets up to 3.5 kb. The kit contains the hot-start Advantage 2 polymerase blend of Titanium Taq DNA Polymerase and a small amount of proofreading enzyme. This blend provides high sensitivity when amplifying a wide range of DNA targets. The kit combines this enzyme blend with a proprietary mix of dNTPs and a specially formulated buffer that work together to achieve 30-fold higher fidelity than that seen with wild-type Taq DNA polymerase..
Overview
- Provides virtually error-free amplification—nearly 30-fold higher fidelity than wild-type Taq
- Produces exceptionally high yields without sacrificing fidelity
- Pre-optimized kit components eliminate the need to optimize reaction conditions
- Generates PCR products (up to 5 kb) that can be cloned into any TA-based vector
Applications
- Cloning/TA-cloning
- Mutation analysis
- Long and accurate PCR
- High-fidelity PCR amplification
Amplification with the Advantage HF 2 PCR Kit
Amplification with the Advantage HF 2 PCR Kit. Panel A. Fidelity (accuracy) of Taq, Advantage 2 Polymerase Mix, and Advantage HF 2 PCR Kit were measured as previously described (1, 2) in a genetic screen. Fidelity of the Advantage enzyme mixes was normalized to Taq, which produced 0.66 errors per 1,000 bp of amplified sequence after 25 PCR cycles. Advantage 2 produced 0.24 errors per 1,000 bp and Advantage HF 2 produced 0.023 errors per 1,000 bp. Panel B. Amplification of a wide size range of cDNA and genomic fragments. Lanes 1–3: amplification of several fragments (0.5–1.8 kb) of the human cardiac beta-myosin chain from human genomic DNA. ;Lanes 4–6: amplification of several size fragments (1.0–3.5 kb) of the BPTI gene from calf thymus DNA. Lanes 7–8: amplification from Marathon-Ready Human Placenta cDNA (Cat. No. 639311). Lane 7: 1.2-kb beta-actin fragment. Lane 8: 1.3-kb TFR fragment. Lane M: 1-kb DNA size marker.
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Amplification with the Advantage HF 2 PCR Kit
Amplification with the Advantage HF 2 PCR Kit. Panel A. Fidelity (accuracy) of Taq, Advantage 2 Polymerase Mix, and Advantage HF 2 PCR Kit were measured as previously described (1, 2) in a genetic screen. Fidelity of the Advantage enzyme mixes was normalized to Taq, which produced 0.66 errors per 1,000 bp of amplified sequence after 25 PCR cycles. Advantage 2 produced 0.24 errors per 1,000 bp and Advantage HF 2 produced 0.023 errors per 1,000 bp. Panel B. Amplification of a wide size range of cDNA and genomic fragments. Lanes 1–3: amplification of several fragments (0.5–1.8 kb) of the human cardiac beta-myosin chain from human genomic DNA. ;Lanes 4–6: amplification of several size fragments (1.0–3.5 kb) of the BPTI gene from calf thymus DNA. Lanes 7–8: amplification from Marathon-Ready Human Placenta cDNA (Cat. No. 639311). Lane 7: 1.2-kb beta-actin fragment. Lane 8: 1.3-kb TFR fragment. Lane M: 1-kb DNA size marker.
