Advantage 2 PCR Kit
Advantage 2 PCR Kit
Brand: Takara Bio.
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Advantage 2 PCR Kit
Advantage 2 PCR Kit
The Advantage 2 PCR Kit includes our Advantage 2 Polymerase Mix (which comes with two optimized buffers), dNTPs, and PCR controls. Our Advantage 2 Polymerase mix is a proven balance of high yield and high fidelity for cDNA amplification and library construction. With 3X higher fidelity than regular Taq, it can readily amplify a wide range of DNA templates, including long templates up to 18 kb and complex genomic DNA up to 6 kb.
Overview
- Unique blend of enzymes provides nearly error-free amplification—almost 3-fold higher fidelity than wild-type Taq
- Exceptionally high yields and sensitivity with almost any target DNA up to 5 kb
- Pre-optimized buffer eliminates the need to troubleshoot reaction conditions
- Integrated hot-start Ab allows room temperature reaction setup and increased specificity
- Available in a lyophilized format as High Fidelity PCR EcoDry Premix, which is convenient for room-temperature shipment and storage
Applications
- Long and accurate PCR
- Preparative PCR
- RACE PCR
- cDNA synthesis/amplification
- cDNA subtraction
- Hot-start PCR
Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix
Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix. 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker
Amplification of various large templates using Advantage 2 Polymerase Mix
Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker.
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Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix
Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix. 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker
Amplification of various large templates using Advantage 2 Polymerase Mix
Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker.
