Adeno-X Rapid Titer Kit
Adeno-X Rapid Titer Kit
Brand: Takara Bio.
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Adeno-X Rapid Titer Kit
The Adeno-X Rapid Titer Kit takes advantage of production of viral hexon proteins for the titration of adenoviral stocks in 48 hours. A viral stock is tested by making serial dilutions, infecting HEK 293 cells, and 48 hours later, fixing and staining those cells with the Mouse Anti-Hexon Antibody. The signal is detected with a Rat Anti-Mouse Antibody conjugated to horseradish peroxidase (HRP) and developed with metal-enhanced 3,3'- Diaminobenzidine tetrahydrochloride (DAB).
Overview
- Obtain a functional titer in 4 hours using either the Adeno-X Rapid Titer Kit (hexon detection) or Adeno-X qPCR Titration Kit (genome detection)
- More precise and less time-consuming than a plaque or end-point dilution assay
Flowchart of the procedures used for titrating adenoviral DNA with the Adeno-X qPCR Titration Kit
Flowchart of the procedures used for titrating adenoviral DNA with the Adeno-X qPCR Titration Kit.
Adeno-X Rapid Titer Kit protocol
Adeno-X Rapid Titer Kit protocol.
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Flowchart of the procedures used for titrating adenoviral DNA with the Adeno-X qPCR Titration Kit
Flowchart of the procedures used for titrating adenoviral DNA with the Adeno-X qPCR Titration Kit.
Adeno-X Rapid Titer Kit protocol
Adeno-X Rapid Titer Kit protocol.
