Adenovirus Purification Kits
Adenovirus Purification Kits
Brand: Takara Bio.
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Adenovirus Purification Kits
Purify adenovirus quickly and easily with our adenovirus purification kits! These kits are chromatography-based systems that enable you to purify amplified adenovirus directly from the cell pellet in less than 1.5 hours. You simply pellet the cells when the cytopathic effect (CPE) is nearly complete, lyse the cells, and purify the virus.
Our Adeno-X Maxi Purification Kit has a wide range of viral capacities, allowing you to scale up or down without difficulty. The kit delivers as many as 1 x 1012 adenoviral particles and provides all of the buffers and materials necessary for purification. This same technology is also available in our convenient Adeno-X Mega Purification Kits with which you can produce and purify up to 1013 highly infectious viral particles (up to 3 x 1011 IFU).
Overview
- Purify up to 1013 adenovirus particles in 90 min
- Use lysate from as many as 5 x 15 cm plates (maxi kit) or 25 x 15 cm plates (mega kit)
- Easy-to-use adenovirus purification assembly
- No CsCl or ultracentrifugation required
NOTE: For the mega kit, we recommend the use of a syringe pump (e.g., Genie Plus from Kent Scientific) to to drive the clarified lysate through the three-tiered purification cartridge.
Overview of the Adeno-X Maxi Purification Kit protocol
Overview of the Adeno-X Maxi Purification Kit protocol.
The filtration apparatus of the Adeno-X adenovirus purification kits are preassembled and includes a one-way valve to simplify the purification process
The filtration apparatus of the Adeno-X adenovirus purification kits are preassembled and includes a one-way valve to simplify the purification process. The one-way valve eliminates the need for repeated syringe removals when binding and washing. The adenoviral stock is brought into the syringe through the valve and pushed out through the purification filter cartridge by the syringe plunger.
Adenovirus is purified directly from the cell pellet of HEK 293 cells
Adenovirus is purified directly from the cell pellet of HEK 293 cells. Starting from the second day after infection, the vast majority of virus is found inside the cell, not in the supernatant. Therefore, combining the supernatant with the cell pellet (as with competitor protocols) unnecessarily complicates the protocol without increasing the amount of virus purified.
Adenovirus purification with high yield
Adenovirus purification with high yield. HEK-293 cells were seeded and infected with adenovirus and harvested when the cytopathic effect was nearly complete. The resulting adenovirus was then purified according to the Adeno-X Maxi Purification Kit protocol and total IFU was determined for each fraction (crude, flowthrough, wash, and eluate). 72% of the starting material was recovered in the eluate as purified virus. The process was efficient and fast, allowing us to purify 4.68 x 1010 adenoviral particles in one hour.
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Overview of the Adeno-X Maxi Purification Kit protocol
Overview of the Adeno-X Maxi Purification Kit protocol.
The filtration apparatus of the Adeno-X adenovirus purification kits are preassembled and includes a one-way valve to simplify the purification process
The filtration apparatus of the Adeno-X adenovirus purification kits are preassembled and includes a one-way valve to simplify the purification process. The one-way valve eliminates the need for repeated syringe removals when binding and washing. The adenoviral stock is brought into the syringe through the valve and pushed out through the purification filter cartridge by the syringe plunger.
Adenovirus is purified directly from the cell pellet of HEK 293 cells
Adenovirus is purified directly from the cell pellet of HEK 293 cells. Starting from the second day after infection, the vast majority of virus is found inside the cell, not in the supernatant. Therefore, combining the supernatant with the cell pellet (as with competitor protocols) unnecessarily complicates the protocol without increasing the amount of virus purified.
Adenovirus purification with high yield
Adenovirus purification with high yield. HEK-293 cells were seeded and infected with adenovirus and harvested when the cytopathic effect was nearly complete. The resulting adenovirus was then purified according to the Adeno-X Maxi Purification Kit protocol and total IFU was determined for each fraction (crude, flowthrough, wash, and eluate). 72% of the starting material was recovered in the eluate as purified virus. The process was efficient and fast, allowing us to purify 4.68 x 1010 adenoviral particles in one hour.
