Adenoviral System 3 (Universal)
Adenoviral System 3 (Universal)
Brand: Takara Bio.
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Adenoviral System 3 (Universal)
Adeno-X Adenoviral System 3 (Universal) provides the components necessary to place an expression cassette of your choice in an adenoviral vector format. The system provides a perfect platform for the tissue-specific expression of your gene of interest, or for the efficient delivery of shRNA or microRNA. pAdenoX-PRLS (included in the vector set) is a prelinearized adenoviral vector that is ready for the insertion of your expression cassette via our In-Fusion HD PCR Cloning technology. Simply PCR-amplify the expression cassette and combine it with pAdenoX-PRLS in an In-Fusion HD Cloning reaction. In-Fusion HD Cloning is fast, simple, precise and efficient, making Adeno-X Adenoviral System 3 the most advanced commercially available adenoviral gene delivery tool.
Overview
- Cloning into adenovirus is as simple and rapid as into any plasmid
- Clone directly into the pAdenoX vector; no shuttle vector required
- Obtain >90% cloning efficiency in just 2–3 days (other systems take 8 days minimum)
- Highly flexible formats—use an existing expression cassette or create one without subcloning
- Transduce dividing and non-dividing cells
- Very high titer, high expression, broad host range
- Tet-On 3G inducible adenoviral system is also available
The universal Adeno-X adenoviral expression systems contain vectors that lack a promoter and polyA signal in the cloning site
The universal Adeno-X adenoviral expression systems contain vectors that lack a promoter and polyA signal in the cloning site. You can either clone an expression cassette from a pre-existing construct into the vector (Panel A) or create a new one using multiple fragment In-Fusion HD Cloning (Panel B).
Adeno-X adenoviral system 3 is available in seven formats, including the most advanced tetracycline-inducible expression system, constitutive expression systems with or without fluorescent reporters, and universal systems that allow you to clone and express any entire expression cassette of your choice
Adeno-X Adenoviral System 3 is available in seven formats, including the most advanced tetracycline inducible expression system, constitutive expression systems with or without fluorescent reporters, and universal systems that allow you to clone and express any entire expression cassette of your choice.
Constructing recombinant adenovirus with In-Fusion Cloning technology
Constructing recombinant adenovirus with In-Fusion Cloning technology. DNA sequences can be rapidly transferred as PCR products to any pAdenoX vector using the In-Fusion cloning method. In this example, your gene of interest is amplified with 15 bp extensions that are homologous to the ends of the linearized adenoviral vector. The PCR product is then purified and mixed with the linearized adenoviral vector of choice in the In-Fusion reaction. Following the reaction, a portion of the mixture is transformed into E. coli (Stellar Competent Cells) and screened. Once a PCR-positive clone is identified, the recombinant pAdenoX vector is amplified, purified, and subsequently linearized with the restriction enzyme PacI, then transfected into Adeno-X 293 cells for viral rescue and amplification. Adeno-X GoStix can be used to determine the status of adenovirus rescue.
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The universal Adeno-X adenoviral expression systems contain vectors that lack a promoter and polyA signal in the cloning site
The universal Adeno-X adenoviral expression systems contain vectors that lack a promoter and polyA signal in the cloning site. You can either clone an expression cassette from a pre-existing construct into the vector (Panel A) or create a new one using multiple fragment In-Fusion HD Cloning (Panel B).
Adeno-X adenoviral system 3 is available in seven formats, including the most advanced tetracycline-inducible expression system, constitutive expression systems with or without fluorescent reporters, and universal systems that allow you to clone and express any entire expression cassette of your choice
Adeno-X Adenoviral System 3 is available in seven formats, including the most advanced tetracycline inducible expression system, constitutive expression systems with or without fluorescent reporters, and universal systems that allow you to clone and express any entire expression cassette of your choice.
Constructing recombinant adenovirus with In-Fusion Cloning technology
Constructing recombinant adenovirus with In-Fusion Cloning technology. DNA sequences can be rapidly transferred as PCR products to any pAdenoX vector using the In-Fusion cloning method. In this example, your gene of interest is amplified with 15 bp extensions that are homologous to the ends of the linearized adenoviral vector. The PCR product is then purified and mixed with the linearized adenoviral vector of choice in the In-Fusion reaction. Following the reaction, a portion of the mixture is transformed into E. coli (Stellar Competent Cells) and screened. Once a PCR-positive clone is identified, the recombinant pAdenoX vector is amplified, purified, and subsequently linearized with the restriction enzyme PacI, then transfected into Adeno-X 293 cells for viral rescue and amplification. Adeno-X GoStix can be used to determine the status of adenovirus rescue.
