Adeno-X GoStix
Adeno-X GoStix are designed to instantly confirm the presence of adenovirus in cell culture supernatants or lysates. The test detects adenoviral hexon proteins and can be used to determine if infectious virus was produced post-transfection, or for selecting the best time to harvest your virus. The included Hexon Control provides confirmation of GoStix test function.
Overview
- More precise and less time-consuming than a plaque or end-point dilution assay
- Obtain a qualitative measurement of adenovirus in 10 minutes using Adeno-X GoStix
- Use Adeno-X GoStix to quickly determine the optimal harvest time
Early detection of adenovirus during rescue
Early detection of adenovirus during rescue. Panel A. The Adeno-X GoStix protocol takes only a couple of minutes to indicate the presence of adenovirus particles. Supernatant from transfected cells is placed on the cassette, followed by chase buffer. Panel B. Adeno-X GoStix were used to confirm virus production during the rescue procedure. PacI-digested adenovirus expressing ZsGreen1 was transfected into HEK 293 cells, which were monitored for the emergence of cytopathic effects by phase and fluorescence microscopy (middle row). 20 μl of culture medium was applied to the Adeno-X GoStix on each day (bottom row). Four days post-transfection, Adeno-X GoStix were able to detect virus in culture supernatants containing plaques that were only weakly perceptible under phase microscopy and confirmed by fluorescence microscopy.
Rapid adenovirus detection during amplification
Rapid adenovirus detection during amplification. Panel A. Adenovirus expressing ZsGreen1 was amplified in HEK293 cells. 48 hr postinfection, and each day thereafter, the supernatant and the cell pellet were harvested, and titers were quantified by counting fluorescent cells in an infectious unit (IFU) assay. The increase in virus level in the cell pellets was mirrored by a proportional increase in the amount of virus collected from the culture supernatant. The amount of virus in the supernatant remained < 10% of the total virus output. Panel B. Supernatant serves as a clear indicator of total yields. In addition to IFU determination, 20 μl of each sample was applied to an Adeno-X GoStix. The increase in band intensity paralleled the increase in virus production observed by infectious unit titer.
Use Adeno-X GoStix to instantly confirm the presence of adenovirus in cell culture supernatants or lysates
Use Adeno-X GoStix to instantly confirm the presence of adenovirus in cell culture supernatants or lysates. The test, which detects adenoviral hexon proteins, requires just 20 µl of adenoviral supernatant to determine if infectious virus was produced posttransfection, or to select the best time to harvest your virus following amplification. The included Hexon Control (bottom) confirms GoStix test function.
What to expect when measuring viral titer with Lenti-X or Adeno-X GoStix
What to expect when measuring viral titer with Lenti-X or Adeno-X GoStix. The control band (C) will always be detected, the test band shows (T) positive only you if there is enough virus in your sample.
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Early detection of adenovirus during rescue
Early detection of adenovirus during rescue. Panel A. The Adeno-X GoStix protocol takes only a couple of minutes to indicate the presence of adenovirus particles. Supernatant from transfected cells is placed on the cassette, followed by chase buffer. Panel B. Adeno-X GoStix were used to confirm virus production during the rescue procedure. PacI-digested adenovirus expressing ZsGreen1 was transfected into HEK 293 cells, which were monitored for the emergence of cytopathic effects by phase and fluorescence microscopy (middle row). 20 μl of culture medium was applied to the Adeno-X GoStix on each day (bottom row). Four days post-transfection, Adeno-X GoStix were able to detect virus in culture supernatants containing plaques that were only weakly perceptible under phase microscopy and confirmed by fluorescence microscopy.
Rapid adenovirus detection during amplification
Rapid adenovirus detection during amplification. Panel A. Adenovirus expressing ZsGreen1 was amplified in HEK293 cells. 48 hr postinfection, and each day thereafter, the supernatant and the cell pellet were harvested, and titers were quantified by counting fluorescent cells in an infectious unit (IFU) assay. The increase in virus level in the cell pellets was mirrored by a proportional increase in the amount of virus collected from the culture supernatant. The amount of virus in the supernatant remained < 10% of the total virus output. Panel B. Supernatant serves as a clear indicator of total yields. In addition to IFU determination, 20 μl of each sample was applied to an Adeno-X GoStix. The increase in band intensity paralleled the increase in virus production observed by infectious unit titer.
Use Adeno-X GoStix to instantly confirm the presence of adenovirus in cell culture supernatants or lysates
Use Adeno-X GoStix to instantly confirm the presence of adenovirus in cell culture supernatants or lysates. The test, which detects adenoviral hexon proteins, requires just 20 µl of adenoviral supernatant to determine if infectious virus was produced posttransfection, or to select the best time to harvest your virus following amplification. The included Hexon Control (bottom) confirms GoStix test function.
What to expect when measuring viral titer with Lenti-X or Adeno-X GoStix
What to expect when measuring viral titer with Lenti-X or Adeno-X GoStix. The control band (C) will always be detected, the test band shows (T) positive only you if there is enough virus in your sample.
