Effect of DNA methylation: This enzyme is capable of cleaving TC5mCGGA, but its rate is about 1/75. Otherwise, enzyme activity is affected by dam methylase
This enzyme is capable of cleaving TC5mCGGA, but its rate is about 1/75. Otherwise, enzyme activity is affected by dam methylase depending on the sequence following the recognition site.
Cleavage of pBR322: commercially available pBR322 is substantially undigested because the adenine residues in the recognition site (TCCGGA) are methylated by dam methylase
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
Effect of DNA methylation: This enzyme is capable of cleaving TC5mCGGA, but its rate is about 1/75. Otherwise, enzyme activity is affected by dam methylase
This enzyme is capable of cleaving TC5mCGGA, but its rate is about 1/75. Otherwise, enzyme activity is affected by dam methylase depending on the sequence following the recognition site.
Cleavage of pBR322: commercially available pBR322 is substantially undigested because the adenine residues in the recognition site (TCCGGA) are methylated by dam methylase
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
