AAVpro Purification Kit (AAV2)
AAVpro Purification Kit (AAV2)
Brand: Takara Bio.
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AAVpro Purification Kit (AAV2)
AAVpro Purification Kit (AAV2)
The purity and titer of the AAV vector is an important for achieving stable and effective introduction of genes into individual animals and cultured cells. Methods such as CsCl concentration gradient ultracentrifugation and iodixanol ultracentrifugation are commonly used to purify AAV2 vectors, but such methods are time-consuming and require careful technique to obtain high yields. The AAVpro Purification Kit (AAV2) allows for simple and fast (~4 hours) AAV2 particle purification from AAV2-producing cells.
Overview
- Kits include all reagents needed to purify AAV particles from vector-producing cells
- Purification yields highly pure AAV particles that can be used directly for in vivo transduction or transduction of cultured cells
- AAVpro Purification Kit (AAV2) is a two-prep kit and does not include Cryonase Cold-Active Nuclease which must be purchased separately
Overview of AAV2 purification using the AAVpro Purification Kit (AAV2)
Overview of AAV2 purification using the AAVpro Purification Kit (AAV2).
Recovery, purity, and infectivity of purified AAV2 particles
Recovery, purity, and infectivity of purified AAV2 particles. AAV2 particles were purified with each company’s purification kit from virus-producing cells. The yield of each purified AAV2 vector was evaluated by real-time PCR (Panel A), and the purity of each purified AAV2 vector was evaluated by SDS-PAGE using 1 x 109 vg per lane (Panel B). Infectivity was measured by infection of HT1080 cells with purified AAV2 particles at 2,500 vg/cell and subsequent measurement of ZsGreen expression (Panel C).
References
Büning, H. and Srivastava, A. Capsid Modifications for Targeting and Improving the Efficacy of AAV Vectors. Mol. Ther. Methods Clin. Dev. 12, 248–265 (2019).
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Overview of AAV2 purification using the AAVpro Purification Kit (AAV2)
Overview of AAV2 purification using the AAVpro Purification Kit (AAV2).
Recovery, purity, and infectivity of purified AAV2 particles
Recovery, purity, and infectivity of purified AAV2 particles. AAV2 particles were purified with each company’s purification kit from virus-producing cells. The yield of each purified AAV2 vector was evaluated by real-time PCR (Panel A), and the purity of each purified AAV2 vector was evaluated by SDS-PAGE using 1 x 109 vg per lane (Panel B). Infectivity was measured by infection of HT1080 cells with purified AAV2 particles at 2,500 vg/cell and subsequent measurement of ZsGreen expression (Panel C).
