6xHis mAb-HRP Conjugate
6xHis mAb-HRP Conjugate
Brand: Takara Bio.
In stock
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6xHis mAb-HRP Conjugate
A monoclonal antibody purified from mouse ascites fluid and conjugated to horseradish peroxidase. The antibody can be used to confirm and/or quantify 6xHis-tagged protein expression by Western blot, ELISA, and immunohistochemistry assays.
Overview
- 6xhis monoclonal antibody: detects as little as 1 ng of 6xhis-tagged proteins
- 6xhis monoclonal antibody-HRP conjugate: has high sensitivity and detection does not require a 2° antibody
- 6xHN polyclonal antibody: detects the 6xHN tag and can be used for Western blotting and ELISA
Applications
Detection via:
- Western Blot
- IP
- ELISA
6xHis Monoclonal Antibody detects 6xHis-LacZ
6xHis Monoclonal Antibody detects 6xHis-LacZ. The protein was purified from lysate on TALON Resin by washing in 10 mM imidazole, and then eluting in 150 mM imidazole buffer. Panel A. IMAC purification fractions of 6xHis-LacZ on TALON Resin were analyzed by SDS-PAGE. Panel B. Albumin-free 6xHis Monoclonal Antibody was used to detect the protein on a Western blot.
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6xHis Monoclonal Antibody detects 6xHis-LacZ
6xHis Monoclonal Antibody detects 6xHis-LacZ. The protein was purified from lysate on TALON Resin by washing in 10 mM imidazole, and then eluting in 150 mM imidazole buffer. Panel A. IMAC purification fractions of 6xHis-LacZ on TALON Resin were analyzed by SDS-PAGE. Panel B. Albumin-free 6xHis Monoclonal Antibody was used to detect the protein on a Western blot.
