Adeno-X 293 Cell Line
Adeno-X 293 Cell Line
Brand: Takara Bio.
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Adeno-X 293 Cell Line
The Adeno-X 293 Cell Line is a low-passage transformed human embryonic kidney cell line for the production of adenovirus stocks. Adenovirus stocks can be produced by either transfecting this cell line with a linearized adenoviral vector (such as the linear vectors supplied with any Adeno-X System 3) or infecting with a viable adenovirus seed stock. The slower growth rate exhibited by this line reduces cell death due to overconfluence, and therefore facilitates more efficient rescue and amplification. These cells also possess strong adherence and are highly transfectable.
Overview
- Low-passage HEK293 clone for packaging adenovirus
- Highly transfectable
- Strongly adherent compared to most laboratory strains
- Slower growth rate facilitates more efficient adenovirus rescue and amplification
Constructing recombinant adenovirus with In-Fusion Cloning technology
Constructing recombinant adenovirus with In-Fusion Cloning technology. DNA sequences can be rapidly transferred as PCR products to any pAdenoX vector using the In-Fusion cloning method. In this example, your gene of interest is amplified with 15 bp extensions that are homologous to the ends of the linearized adenoviral vector. The PCR product is then purified and mixed with the linearized adenoviral vector of choice in the In-Fusion reaction. Following the reaction, a portion of the mixture is transformed into E. coli (Stellar Competent Cells) and screened. Once a PCR-positive clone is identified, the recombinant pAdenoX vector is amplified, purified, and subsequently linearized with the restriction enzyme PacI, then transfected into Adeno-X 293 cells for viral rescue and amplification. Adeno-X GoStix can be used to determine the status of adenovirus rescue.
Phase contrast microscopy image of the Adeno-X 293 Cell Line
Phase contrast microscopy image of the Adeno-X 293 Cell Line.
During adenovirus amplification and rescue, Adeno-X 293 cells typically remain intact but round up and may detach from the plate
During adenovirus amplification and rescue, Adeno-X 293 cells typically remain intact but round up and may detach from the plate. These changes are collectively referred to as the cytopathic effect (CPE). These images show CPE following transfection of Adeno-X ZsGreen1 DNA into the Adeno-X 293 Cell Line.
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Constructing recombinant adenovirus with In-Fusion Cloning technology
Constructing recombinant adenovirus with In-Fusion Cloning technology. DNA sequences can be rapidly transferred as PCR products to any pAdenoX vector using the In-Fusion cloning method. In this example, your gene of interest is amplified with 15 bp extensions that are homologous to the ends of the linearized adenoviral vector. The PCR product is then purified and mixed with the linearized adenoviral vector of choice in the In-Fusion reaction. Following the reaction, a portion of the mixture is transformed into E. coli (Stellar Competent Cells) and screened. Once a PCR-positive clone is identified, the recombinant pAdenoX vector is amplified, purified, and subsequently linearized with the restriction enzyme PacI, then transfected into Adeno-X 293 cells for viral rescue and amplification. Adeno-X GoStix can be used to determine the status of adenovirus rescue.
Phase contrast microscopy image of the Adeno-X 293 Cell Line
Phase contrast microscopy image of the Adeno-X 293 Cell Line.
During adenovirus amplification and rescue, Adeno-X 293 cells typically remain intact but round up and may detach from the plate
During adenovirus amplification and rescue, Adeno-X 293 cells typically remain intact but round up and may detach from the plate. These changes are collectively referred to as the cytopathic effect (CPE). These images show CPE following transfection of Adeno-X ZsGreen1 DNA into the Adeno-X 293 Cell Line.
