NucleoMag DNA/RNA Water
NucleoMag DNA/RNA Water
Brand: MACHEREY-NAGEL
In stock
SKU
NucleoMag DNA/RNA Water
Applications
- Microbial DNA and RNA from filtered water and air samples
- Manual and automated procedures at room temperature without reducing agents
- Typical downstream applications: qPCR, qRT-PCR, NGS
Features
- Suitable for diverse salty and fresh water samples, ranging from turbid to clear as well as with air filters
- Minimized inhibition for reliable results
- Compatible with a variety of filters and filtration systems
- Can be additionally combined with two different sizes of ceramic bead tubes for optimal sample lysis
Specifications
- Technology: Magnetic bead technology
- Format: Highly reactive superparamagnetic beads
- Processing: Manual or automated
- Sample material: Water and air samples
- Fragment size: 300 bp–approx. 50 kbp
- Elution volume: 50–200 µL
- Preparation time: 40 min for KingFisher® Flex (excl. filtration and sample lysis)
- Theoretical binding capacity: 0.4 µg/µL beads
Microbial nucleic acids from water and air
The new NucleoMag® DNA/RNA Water enables manual or au‑ tomated detection of either DNA, RNA, or both molecules in parallel from a range of water samples, spanning from turbid to clear, as well as from air filters. Simultaneous detection of DNA and RNA in the same sample enables fast and accurate determination of presence of total microbes (through DNA) and living microorganisms (through RNA) in the same sample. NucleoMag® DNA/RNA is compatible with a variety of filters and filtration systems, including conventional round filters (e. g., 25 mm to 47 mm) from various materials as well as with cartridge filters (such as Sterivex™). This makes NucleoMag® DNA/RNA Water ideal for microbial metagenomics, environ‑ mental microbiology as well as for estimation of water quality through determination of the microbial load of the sample.
Procedure
Efficient detection for different water and air samples
Various water samples and an air sample were filtered and the extracted DNA was analyzed by PCR. Microbial DNA could be efficiently measured for all of the samples, demonstrating the versatility of the NucleoMag® DNA/RNA Water kit.
Compatibility with different filtration systems
A qPCR was performed with nucleic acids isolated from round filters and a filtration cartridge system, demonstrating reliable results across different fil‑ tration systems.
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Microbial nucleic acids from water and air
The new NucleoMag® DNA/RNA Water enables manual or au‑ tomated detection of either DNA, RNA, or both molecules in parallel from a range of water samples, spanning from turbid to clear, as well as from air filters. Simultaneous detection of DNA and RNA in the same sample enables fast and accurate determination of presence of total microbes (through DNA) and living microorganisms (through RNA) in the same sample. NucleoMag® DNA/RNA is compatible with a variety of filters and filtration systems, including conventional round filters (e. g., 25 mm to 47 mm) from various materials as well as with cartridge filters (such as Sterivex™). This makes NucleoMag® DNA/RNA Water ideal for microbial metagenomics, environ‑ mental microbiology as well as for estimation of water quality through determination of the microbial load of the sample.
Procedure
Efficient detection for different water and air samples
Various water samples and an air sample were filtered and the extracted DNA was analyzed by PCR. Microbial DNA could be efficiently measured for all of the samples, demonstrating the versatility of the NucleoMag® DNA/RNA Water kit.
Compatibility with different filtration systems
A qPCR was performed with nucleic acids isolated from round filters and a filtration cartridge system, demonstrating reliable results across different fil‑ tration systems.
