NucleoMag DNA/RNA Water

Microbial nucleic acids from water and air

The new NucleoMag® DNA/RNA Water enables manual or au‑ tomated detection of either DNA, RNA, or both molecules in parallel from a range of water samples, spanning from turbid to clear, as well as from air filters. Simultaneous detection of DNA and RNA in the same sample enables fast and accurate determination of presence of total microbes (through DNA) and living microorganisms (through RNA) in the same sample. NucleoMag® DNA/RNA is compatible with a variety of filters and filtration systems, including conventional round filters (e. g., 25 mm to 47 mm) from various materials as well as with cartridge filters (such as Sterivex™). This makes NucleoMag® DNA/RNA Water ideal for microbial metagenomics, environ‑ mental microbiology as well as for estimation of water quality through determination of the microbial load of the sample.

Procedure

Efficient detection for different water and air samples

Various water samples and an air sample were filtered and the extracted DNA was analyzed by PCR. Microbial DNA could be efficiently measured for all of the samples, demonstrating the versatility of the NucleoMag® DNA/RNA Water kit.

Compatibility with different filtration systems

A qPCR was performed with nucleic acids isolated from round filters and a filtration cartridge system, demonstrating reliable results across different fil‑ tration systems.