Premix Ex Taq DNA Polymerase (Perfect Real Time)
Premix Ex Taq DNA Polymerase (Perfect Real Time)
Brand: Takara Bio.
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Premix Ex Taq DNA Polymerase (Perfect Real Time)
Premix Ex Taq DNA polymerase for real-time PCR
Premix Ex Taq DNA Polymerase (Perfect Real Time) is a 2X RT-PCR kit specifically designed for fast and sensitive real-time PCR via either intercalating green dye real-time PCR (qPCR) or probe-based qPCR assays. TB Green dye and probes for probe/5' nuclease-based assays are not included in this RT-PCR kit. The Premix Ex Taq DNa Polymerase (Perfect RealTime) kit consists of our high-fidelity and high-performance Takara Ex Taq Hot Start polymerase and a real-time buffer that ensures superior specificity and increased amplification efficiency during RT-PCR (qPCR). Antibody-mediated hot-start technology prevents nonspecific amplification due to mispriming and/or the formation of primer dimers during room-temperature reaction assembly. Once the Taq antibody-polymerase complex is denatured during the first cycling step, the Premix Ex Taq polymerase can initiate DNA synthesis.
Two tubes of ROX Reference Dyes are supplied as separate components of this RT-PCR kit. ROX Dye is a convenient internal reference standard that may be used to normalize signal variances that occur as a consequence of non-PCR-related fluorescence fluctuations across different wells or over time.
Premix Ex Taq DNA Polymerase (Perfect Real Time) provides superior specificity, performance, and amplification yield during RT-PCR on all major real-time instruments.
Overview
- High specificity and increased amplification efficiency for qPCR
- Sensitivity down to 10 copies for intercalating green dye or probe detection
- Compatibility with 5' nuclease assays and with TB Green dye
- Compatibility with many commonly used real-time PCR instruments
Applications
- Real-time PCR using probe-based qPCR assays or intercalating green dye
Compatible cyclers
- SmartCycler, Light Cycler, ABI PRISM 7000/7700/7900 HT, Applied Biosystems 7300/7500, iCycler, MJ Opticon, Stratagene MX 3000
- A dynamic range of 7–8 orders of magnitude using TB Green dye and 10 orders of magnitude using probes can be expected with Premix Ex Taq DNA Polymerase (Perfect Real Time). Expected sensitivity is as low as 10 copies for both TB Green and probe-based qPCR assays.
Amplification Curve (top panel) and Standard Curve (bottom) for Premix Ex Taq (Perfect Real Time) using the TaqMan Gene Expression Assay on the Applied Biosystems 7500 Real-Time PCR System
Amplification Curve (top panel) and Standard Curve (bottom) for Premix Ex Taq (Perfect Real Time) using the TaqMan Gene Expression Assay on the Applied Biosystems 7500 Real-Time PCR System.
Product citations
Hayama E. et al. Analysis of Voltage-Gated Potassium Channel β1 Subunits in the Porcine Neonatal Ductus Arteriosus. Pediatr. Res. 59(2):167–174 (2006).
Iwahara, K. et al. Detection of cfxA and cfxA2, the β-lactamase genes of Prevotella spp., in clinical samples from dentoalveolar infection by real-time PCR. J. Clin. Microbiol. 44(1):172–176 (2006).
Jordan, E.N. et al. Real-time polymerase chain reaction for detecting bacterial DNA directly from blood of neonates being evaluated for sepsis. J. Mol. Diagnost. 7(5):575–581 (2005).
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Amplification Curve (top panel) and Standard Curve (bottom) for Premix Ex Taq (Perfect Real Time) using the TaqMan Gene Expression Assay on the Applied Biosystems 7500 Real-Time PCR System
Amplification Curve (top panel) and Standard Curve (bottom) for Premix Ex Taq (Perfect Real Time) using the TaqMan Gene Expression Assay on the Applied Biosystems 7500 Real-Time PCR System.
