Premix Ex Taq DNA polymerase for real-time PCR
Premix Ex Taq DNA Polymerase (Perfect Real Time) is a 2X RT-PCR kit specifically designed for fast and sensitive real-time PCR via either intercalating green dye real-time PCR (qPCR) or probe-based qPCR assays. TB Green dye and probes for probe/5′ nuclease-based assays are not included in this RT-PCR kit. The Premix Ex Taq DNa Polymerase (Perfect RealTime) kit consists of our high-fidelity and high-performance Takara Ex Taq Hot Start polymerase and a real-time buffer that ensures superior specificity and increased amplification efficiency during RT-PCR (qPCR). Antibody-mediated hot-start technology prevents nonspecific amplification due to mispriming and/or the formation of primer dimers during room-temperature reaction assembly. Once the Taq antibody-polymerase complex is denatured during the first cycling step, the Premix Ex Taq polymerase can initiate DNA synthesis.
Two tubes of ROX Reference Dyes are supplied as separate components of this RT-PCR kit. ROX Dye is a convenient internal reference standard that may be used to normalize signal variances that occur as a consequence of non-PCR-related fluorescence fluctuations across different wells or over time.
Premix Ex Taq DNA Polymerase (Perfect Real Time) provides superior specificity, performance, and amplification yield during RT-PCR on all major real-time instruments.
Overview
- High specificity and increased amplification efficiency for qPCR
- Sensitivity down to 10 copies for intercalating green dye or probe detection
- Compatibility with 5′ nuclease assays and with TB Green dye
- Compatibility with many commonly used real-time PCR instruments
Applications
- Real-time PCR using probe-based qPCR assays or intercalating green dye
Compatible cyclers
- SmartCycler, Light Cycler, ABI PRISM 7000/7700/7900 HT, Applied Biosystems 7300/7500, iCycler, MJ Opticon, Stratagene MX 3000
- A dynamic range of 7–8 orders of magnitude using TB Green dye and 10 orders of magnitude using probes can be expected with Premix Ex Taq DNA Polymerase (Perfect Real Time). Expected sensitivity is as low as 10 copies for both TB Green and probe-based qPCR assays.
