pCold DNA Cold-shock Expression System
pCold DNA Cold-shock Expression System
Brand: Takara Bio.
In stock
SKU
pCold DNA Cold-shock Expression System
pCold Series
pCold expression vectors offer cold-shock expression technology for high-purity, high-yield protein production.
The pCold series includes several different vectors. Each includes the cold-shock Protein A (cspA) promoter for expression of high-purity, high-yield recombinant protein in E. coli. These vectors selectively induce target protein synthesis at a low temperature (15°C), a condition which suppresses the expression of host proteins and decreases protease activity. This results in high yields of target proteins (60% of intracellular protein). In addition to the cspA promoter, all vectors contain a lac operator, ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and a multiple cloning site (MCS). Four of the vectors contain either a translation enhancing element (TEE), his-tag sequence, and/or Factor Xa cleavage site. The pCold GST vector contains a glutathione S-transferase (GST) sequence and can be used for the expression of proteins with an N-terminal GST tag. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins, and can be used in conjunction with our Chaperone Plasmid Set.
Overview
- Expression of difficult proteins that can not be expressed with the T7 system
- Increased solubility due to expression at reduced temperature
- Increased purity due to repressed expression of host proteins
- When used in conjunction with one of our Chaperone Plasmids, the amount of recoverable soluble protein can be further increased
- Radioisotope labeling: Up to 90% of newly expressed cellular protein is labeled target protein
- Chain length:
- pCold I DNA: 4,407 bp
- pCold II DNA: 4,392 bp
- pCold III DNA: 4,377 bp
- pCold IV DNA: 4,359 bp
- pCold GST DNA: 5,097 bp
Applications
- High-efficiency protein expression using a cold-shock promoter
pCold GST DNA Vector Map
pCold GST DNA Vector Map .
pCold Vector Map
pCold Vector Map.
Multiple cloning site of pCold I DNA (Cat.# 3361).
Multiple cloning site of pCold I DNA (Cat.# 3361).
Multiple cloning site of pCold I DNA (Cat.# 3361).
Multiple cloning site of pCold I DNA (Cat.# 3361).
Multiple cloning site of pCold II DNA (Cat.# 3362)
Multiple cloning site of pCold II DNA (Cat.# 3362).
Multiple cloning site of pCold III DNA (Cat.# 3363)
Multiple cloning site of pCold III DNA (Cat.# 3363).
Multiple cloning site of pCold IV DNA (Cat.# 3364)
Multiple cloning site of pCold IV DNA (Cat.# 3364).
Luis, B.G., et al., Cloning and initial characterization of a family A DNA polymerase from Entamoeba histolytica: A putative mitochondrial DNA Polymerase. FASEB J 21:A1039 (2007).
Kobayashi, H., et al., Significant enhanced expression and solubility of human proteins in Escherichia coli by fusion with Protein S from Myxococcus xanthus. Appl. Envir. Microbiol. 75: 5356–5362 (2009).
Kawakami, R., et al., Gene cloning and characterization of the Very Large NAD-Dependent L-Glutamate Dehydrogenase from the psychrophile Janthinobacterium lividum, isolated from cold soil. J. Bacteriol. 189:5626–5633 (2007).
Eguchi, T., et al., Novel transcription factor-like function of human matrix metalloproteinase 3 regulating the CTGF/CCN2 gene. Mol. Cell. Biol. 28: 2391–2413 (2008).
Tsuge, Y., et al., Deletion of cgR_1596 and cgR_2070, encoding NlpC/P60 proteins, causes a defect in cell separation in Corynebacterium glutamicum. R J. Bacteriol.190:8204–8214 (2008).
Write Your Own Review
pCold GST DNA Vector Map
pCold GST DNA Vector Map .
pCold Vector Map
pCold Vector Map.
Multiple cloning site of pCold I DNA (Cat.# 3361).
Multiple cloning site of pCold I DNA (Cat.# 3361).
Multiple cloning site of pCold I DNA (Cat.# 3361).
Multiple cloning site of pCold I DNA (Cat.# 3361).
Multiple cloning site of pCold II DNA (Cat.# 3362)
Multiple cloning site of pCold II DNA (Cat.# 3362).
Multiple cloning site of pCold III DNA (Cat.# 3363)
Multiple cloning site of pCold III DNA (Cat.# 3363).
Multiple cloning site of pCold IV DNA (Cat.# 3364)
Multiple cloning site of pCold IV DNA (Cat.# 3364).
