One-step, Dye-based RT-qPCR
One-step, Dye-based RT-qPCR
Brand: Takara Bio.
In stock
SKU
One-step, Dye-based RT-qPCR
All RT-PCR steps performed in a single tube, simplifying reaction setup, minimising risk of contamination
One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time)
One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) is designed for intercalator-based, one-step real-time RT-PCR using TB Green. All steps of RT-PCR can be performed in a single tube, allowing a simple procedure and minimizing the risk of contamination. Also, amplified products are monitored in real time, so there is no need to verify by electrophoresis after PCR. This kit is suitable for detection of tiny amounts of RNA such as RNA viruses. This kit uses PrimeScript RTase, which has excellent extendibility and can efficiently synthesize cDNA quickly, and Takara Ex Taq HS, a high-efficiency hot start PCR enzyme, optimized for one-step RT-PCR. This kit achieves a simple and convenient procedure with premixed components.
Overview
- Quantitative real-time PCR enables rapid and accurate analysis of RNA from viral, rare, or precious samples.
- Takara Ex Taq HS is a high-efficiency hot-start PCR enzyme that can be used in RT-PCR.
- The One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) buffer system is optimized for quantitative real-time PCR, allowing efficient amplification and highly sensitive detection.
- One-Step TB Green RT-PCR Buffer IV is a 2X TB Green premix; the premix formulation simplifies reaction mixture preparation.
Applications
- Quantitative real-time PCR
- Intercalating dye-based real-time PCR (qPCR)
- Detection and analysis of small RNA amounts
Compatible real-time PCR instruments
- Thermal Cycler Dice Real Time System
- ABI PRISM 7000/7700, Applied Biosystems 7300/7500 Real-Time PCR System (Applied Biosystems)
- LightCycler (Roche Diagnostics)
One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time)
One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time)
Arita, M., Wakita, T. & Shimizu, H. Characterization of pharmacologically active compounds that inhibit poliovirus and enterovirus 71 infectivity. J. Gen. Virol. 89, 2518-30 (2008).
Murata, T. et al. Involvement of Jun dimerization protein 2 (JDP2) in the maintenance of Epstein-Barr virus latency. J. Biol. Chem. 286, 22007-16 (2011).
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One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time)
