Mighty Cloning Reagent Set (Blunt End)

Takara's Reagent Set for Mighty Cloning Kit (Blunt End) is designed to simply and quickly prepare PCR products for cloning into blunt-ended vectors. Because the blunting and 5'-phosphorylation reactions are done simultaneously, both blunt- and dA-ended products can be prepared in a single reaction. This kit eliminates the need for pre-treatment (such as enzyme inactivation, removal of unused dNTPs and primers, etc.), of PCR products. This kit includes the same ligation reagents as are included in Takara's DNA Ligation Kit (Mighty Mix), which provide simple, quick, and efficient ligation. The kit is suitable for blunt-end cloning of PCR products obtained with PrimeSTAR HS and e2TAK DNA Polymerases.

Applications

Quick and easy preparation of PCR products for cloning into blunt-end vectors

pUC118 Hinc II / BAPpUC118 DNA (BAP-treated) is a useful blunt-end cloning vector, prepared by cleavage with Hinc II, then dephosphorylated by alkaline phosphatase purified from E. coli

pUC118 Hinc II / BAP

pUC118 DNA (BAP-treated) is a useful blunt-end cloning vector, prepared by cleavage with Hinc II, then dephosphorylated by alkaline phosphatase purified from E.coli (BAP). The sequence of DNA cloned into this vector can be verified with M13 primer M4 and RV.

Vector map of pUC118

Cloning site of pUC118pUC118 Hinc II / BAPpUC118 DNA (BAP-treated) is a useful blunt-end cloning vector, prepared by cleavage with Hinc II, then dephosphorylated by alkaline phosphatase purified from E

Cloning site of pUC118

pUC118 Hinc II / BAP

pUC118 DNA (BAP-treated) is a useful blunt-end cloning vector, prepared by cleavage with Hinc II, then dephosphorylated by alkaline phosphatase purified from E. coli (BAP). The sequence of DNA cloned into this vector can be verified with M13 primer M4 and RV.