Xeno-free Human MSC Culture Medium
Xeno-free Human MSC Culture Medium
Brand: Takara Bio.
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Xeno-free Human MSC Culture Medium
Cellartis MSC Xeno-Free Culture Medium is suitable for the maintenance and expansion of human mesenchymal stem cells (MSC) from bone marrow, adipose tissue, or umbilical cord—without plate coating reagents. However, coating cell culture vessels with RetroNectin reagent or human fibronectin can further promote proliferation. This xeno-free medium is ideal for cell therapy research applications such as bone/cartilage diseases, bone marrow transplants/GVHD, cardiovascular disease, autoimmune disease, liver disease, and cancer.
Overview
- Stable MSC phenotype—maintain expression of CD markers and multilinear differentiation ability
- Robust proliferation—grow enough cells for cell therapy research applications
- Flexibility and ease of use—maintain MSCs on regular tissue culture plates without coating reagent, or coat plates with RetroNectin reagent
Applications
- Maintenance and expansion of human MSCs derived from bone marrow, adipose tissue, or umbilical cord
- Ideal for cell therapy research applications such as:
- Bone and cartilage diseases, BMT, and GVHD
- Cardiovascular diseases
- Autoimmune diseases
- Liver diseases
- Cancer
Cellartis xeno-free MSC medium: robust proliferation even without coating
hMSCs derived from bone marrow, adipose and umbilical cord were maintained in Cellartis MSC Xeno-Free Culture Medium or a competitor's xeno-free medium, with and without coating (fibronectin), on regular tissue culture plates. Cells grew better for five passages in Cellartis MSC Xeno-Free Culture Medium than in the competitor's medium. MSCs could not attach on the regular tissue culture plate after two or three passages in the competitor's medium without coating.
Cellartis xeno-free MSC medium maintains healthy MSC phenotype
hMSCs derived from bone marrow (BM), adipose (AD), and umbilical cord (UC) were maintained in Cellartis MSC Xeno-Free Culture Medium or a competitor's xeno-free medium, w/ and w/o coating (fibronectin) on regular tissue culture plates. After both 1 and 5 passages, % of CD90+, CD105+ and CD73+ MSCs are identified by FACS analysis. After 5 passages (24 days), Cellartis MSC Xeno-Free Culture Medium maintained a high percentage of cells that were positive for typical MSC markers.
Cellartis xeno-free MSC medium maintains MSC multilinear differentiation ability
hMSCs derived from bone marrow (Panel A) were successfully differentiated into osteoblast (Panel B), adipocyte (Panel C), and chondrocyte (Panel D) following culture in Cellartis MSC Xeno-Free Culture Medium without coating reagent.
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Cellartis xeno-free MSC medium: robust proliferation even without coating
hMSCs derived from bone marrow, adipose and umbilical cord were maintained in Cellartis MSC Xeno-Free Culture Medium or a competitor's xeno-free medium, with and without coating (fibronectin), on regular tissue culture plates. Cells grew better for five passages in Cellartis MSC Xeno-Free Culture Medium than in the competitor's medium. MSCs could not attach on the regular tissue culture plate after two or three passages in the competitor's medium without coating.
Cellartis xeno-free MSC medium maintains healthy MSC phenotype
hMSCs derived from bone marrow (BM), adipose (AD), and umbilical cord (UC) were maintained in Cellartis MSC Xeno-Free Culture Medium or a competitor's xeno-free medium, w/ and w/o coating (fibronectin) on regular tissue culture plates. After both 1 and 5 passages, % of CD90+, CD105+ and CD73+ MSCs are identified by FACS analysis. After 5 passages (24 days), Cellartis MSC Xeno-Free Culture Medium maintained a high percentage of cells that were positive for typical MSC markers.
Cellartis xeno-free MSC medium maintains MSC multilinear differentiation ability
hMSCs derived from bone marrow (Panel A) were successfully differentiated into osteoblast (Panel B), adipocyte (Panel C), and chondrocyte (Panel D) following culture in Cellartis MSC Xeno-Free Culture Medium without coating reagent.
