Xeno-free Human MSC Culture Medium

Cellartis xeno-free MSC medium: robust proliferation even without coating

hMSCs derived from bone marrow, adipose and umbilical cord were maintained in Cellartis MSC Xeno-Free Culture Medium or a competitor’s xeno-free medium, with and without coating (fibronectin), on regular tissue culture plates. Cells grew better for five passages in Cellartis MSC Xeno-Free Culture Medium than in the competitor’s medium. MSCs could not attach on the regular tissue culture plate after two or three passages in the competitor’s medium without coating.

Cellartis xeno-free MSC medium maintains healthy MSC phenotype

hMSCs derived from bone marrow (BM), adipose (AD), and umbilical cord (UC) were maintained in Cellartis MSC Xeno-Free Culture Medium or a competitor’s xeno-free medium, w/ and w/o coating (fibronectin) on regular tissue culture plates. After both 1 and 5 passages, of CD90+, CD105+ and CD73+ MSCs are identified by FACS analysis. After 5 passages (24 days), Cellartis MSC Xeno-Free Culture Medium maintained a high percentage of cells that were positive for typical MSC markers.

Cellartis xeno-free MSC medium maintains MSC multilinear differentiation ability

hMSCs derived from bone marrow (Panel A) were successfully differentiated into osteoblast (Panel B), adipocyte (Panel C), and chondrocyte (Panel D) following culture in Cellartis MSC Xeno-Free Culture Medium without coating reagent.