TaKaRa Z-Taq DNA Polymerase

Brand	Takara Bio

Takara Z-Taq is characterized by 5-fold greater processivity than Taq DNA Polymerase, resulting in rapid amplification times in PCR applications. Separate tubs of optimzed buffer (Mg2+ plus), and dNTP mix are supplied with the polymerase.

Overview

Reaction speed five times faster than standard Taq DNA polymerase
Amplify human genomic DNA templates up to 17.5 kb, and lambda DNA templates of 20 kb

Applications

Fast PCR

PCR products

PCR products generated with Takara Z-Taq contain a mixture of 3′-A overhangs and blunt ends, allowing >80% cloning efficiency when using T-vectors.

A 1 kb fragment of the G3PDH gene was amplified from 0

A 1 kb fragment of the G3PDH gene was amplified from 0.1, 1, 10, or 100 ng of human placenta DNA. The cycling conditions were 30cycles of 98° C, 5 seconds; 68°C, 20 seconds. After PCR, 5 µl of each reaction mixture was loaded on an agarose gel for electrophoresis. The results indicate that Z-Taq DNA Polymerase could amplify the 1 kb target from as little as 1 ng of template DNA.

Amplification of long products using Takara Z-Taq DNA Polymerase

Amplification of long products using Takara Z-Taq DNA Polymerase.

Long products (7.5 kb, 17.5 kb, 18 kb, or 20 kb) were amplified from human genomic DNA (500 ng/50 µl reaction) or E. coli genomic DNA (100 ng/50 µl reaction) using the following conditions:

[Human 7.5 kb product]

30 cycles of: 98°C, 5 sec.; 68°C, 80 sec.

[E. coli 18 and 20 kb products and human 17.5 kb product]

35 cycles of: 98°C, 5 sec.; 68°C, 180 sec.

All reactions were performed using a TaKaRa PCR Thermal Cycler PERSONAL (discontinued) using the FAST cycling mode. After PCR, 5 µl of the reaction mixture was loaded on an agarose gel for electrophoresis. Takara Bio’s Z-Taq DNA Polymerase efficiently amplified large genomic fragments, including a 20 kb target.

TaKaRa Z-Taq DNA Polymerase

Additional information