TaKaRa Z-Taq DNA Polymerase
TaKaRa Z-Taq DNA Polymerase
Brand: Takara Bio.
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TaKaRa Z-Taq DNA Polymerase
TaKaRa Z-Taq DNA Polymerase
Takara Z-Taq is characterized by 5-fold greater processivity than Taq DNA Polymerase, resulting in rapid amplification times in PCR applications. Separate tubs of optimzed buffer (Mg2+ plus), and dNTP mix are supplied with the polymerase.
Overview
- Reaction speed five times faster than standard Taq DNA polymerase
- Amplify human genomic DNA templates up to 17.5 kb, and lambda DNA templates of 20 kb
Applications
- Fast PCR
PCR products
PCR products generated with Takara Z-Taq contain a mixture of 3'-A overhangs and blunt ends, allowing >80% cloning efficiency when using T-vectors.
A 1 kb fragment of the G3PDH gene was amplified from 0
A 1 kb fragment of the G3PDH gene was amplified from 0.1, 1, 10, or 100 ng of human placenta DNA. The cycling conditions were 30cycles of 98° C, 5 seconds; 68°C, 20 seconds. After PCR, 5 µl of each reaction mixture was loaded on an agarose gel for electrophoresis. The results indicate that Z-Taq DNA Polymerase could amplify the 1 kb target from as little as 1 ng of template DNA.
Amplification of long products using Takara Z-Taq DNA Polymerase
Amplification of long products using Takara Z-Taq DNA Polymerase.
Long products (7.5 kb, 17.5 kb, 18 kb, or 20 kb) were amplified from human genomic DNA (500 ng/50 µl reaction) or E. coli genomic DNA (100 ng/50 µl reaction) using the following conditions:
[Human 7.5 kb product]
30 cycles of: 98°C, 5 sec.; 68°C, 80 sec.
[E. coli 18 and 20 kb products and human 17.5 kb product]
35 cycles of: 98°C, 5 sec.; 68°C, 180 sec.
All reactions were performed using a TaKaRa PCR Thermal Cycler PERSONAL (discontinued) using the FAST cycling mode. After PCR, 5 µl of the reaction mixture was loaded on an agarose gel for electrophoresis. Takara Bio's Z-Taq DNA Polymerase efficiently amplified large genomic fragments, including a 20 kb target.
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A 1 kb fragment of the G3PDH gene was amplified from 0
A 1 kb fragment of the G3PDH gene was amplified from 0.1, 1, 10, or 100 ng of human placenta DNA. The cycling conditions were 30cycles of 98° C, 5 seconds; 68°C, 20 seconds. After PCR, 5 µl of each reaction mixture was loaded on an agarose gel for electrophoresis. The results indicate that Z-Taq DNA Polymerase could amplify the 1 kb target from as little as 1 ng of template DNA.
Amplification of long products using Takara Z-Taq DNA Polymerase
Amplification of long products using Takara Z-Taq DNA Polymerase.
Long products (7.5 kb, 17.5 kb, 18 kb, or 20 kb) were amplified from human genomic DNA (500 ng/50 µl reaction) or E. coli genomic DNA (100 ng/50 µl reaction) using the following conditions:
[Human 7.5 kb product]
30 cycles of: 98°C, 5 sec.; 68°C, 80 sec.
[E. coli 18 and 20 kb products and human 17.5 kb product]
35 cycles of: 98°C, 5 sec.; 68°C, 180 sec.
All reactions were performed using a TaKaRa PCR Thermal Cycler PERSONAL (discontinued) using the FAST cycling mode. After PCR, 5 µl of the reaction mixture was loaded on an agarose gel for electrophoresis. Takara Bio's Z-Taq DNA Polymerase efficiently amplified large genomic fragments, including a 20 kb target.
