SMARTer RACE 5’/3’ Kit
SMARTer RACE 5’/3’ Kit
Brand: Takara Bio.
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SMARTer RACE 5’/3’ Kit
The SMARTer RACE 5'/3' Kit allows the synthesis of first-strand cDNA from poly A+ or total RNA via SMART (Switching Mechanism At 5' End of RNA Template) technology, and facilitates the performance of 5'- and 3'-RACE (Rapid Amplification of cDNA Ends) PCR with the kit's Universal Primer Mix. Our carefully designed, specially-modified SMARTer Oligo preferentially captures the 5' ends of the cDNA during cDNA synthesis. Using this SMARTer Oligo, our procedure enriches cDNA pools for 5' sequences, thus increasing the likelihood you will amplify the entire sequence of your gene.
RACE PCR products are amplified with the provided SeqAmp DNA Polymerase, and cloned into the linearized pRACE vector with In-Fusion HD Cloning. The SMARTer RACE 5'/3' Kit has been improved to accommodate larger RNA input volumes and perform better on challenging targets than the original SMARTer RACE cDNA Amplification Kit. The In-Fusion HD Cloning Kit, NucleoSpin Gel and PCR Clean-Up Kit, and Stellar Competent Cells are included for your convenience in cloning RACE products. Gene-specific RACE primers are supplied by the user.
Overview
SMARTer RACE 5'/3' Kit (Cat. # 634858, 634859)
- Simple kit—easy to use
- SeqAmp DNA Polymerase provides robust PCR performance
- In-Fusion HD technology enables fast, easy cloning of RACE fragments
- Requires only 10 ng of total RNA
- Specific enrichment for either 5' or 3' ends
- No RNA pretreatment required
Applications
- RACE (Rapid Amplification of cDNA Ends)
- Identification of 3'/5' UTRs
- Full-length gene cloning
- Gene cloning and analysis
Overview of the SMARTer RACE 5'/3' Kit workflow
Overview of the SMARTer RACE 5'/3' Kit workflow. Each kit is a complete system, containing the reagents required to recover cloned RACE fragments on the second day.
Comparing products from the new and old SMARTer RACE kits
Comparing products from the new and old SMARTer RACE kits. Primers were designed using the recommendations in each kit's manual. The samples on this gel image represent a wide range of expression values, and each one has a combined exon length of >10 kb. The new kit is much more successful in amplifying strong, single bands across the sample set.
Bertling, W. M., Beier, F. & Reichenberger, E. Determination of 5' ends of specific mRNAs by DNA ligase-dependent amplification. PCR Methods Appl. 3, 95–9 (1993).
Frohman, M. A. Rapid amplification of complementary DNA ends for generation of full-length complementary DNAs: thermal RACE. Methods Enzymol. 218, 340–56 (1993).
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Overview of the SMARTer RACE 5'/3' Kit workflow
Overview of the SMARTer RACE 5'/3' Kit workflow. Each kit is a complete system, containing the reagents required to recover cloned RACE fragments on the second day.
Comparing products from the new and old SMARTer RACE kits
Comparing products from the new and old SMARTer RACE kits. Primers were designed using the recommendations in each kit's manual. The samples on this gel image represent a wide range of expression values, and each one has a combined exon length of >10 kb. The new kit is much more successful in amplifying strong, single bands across the sample set.
