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SmaI
SmaI restriction site:
CCC | GGG
GGG | CCC
Supplied buffer: T+BSA
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 30°C
- Source: Serratia marcescens Sb
- Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: lambda DNA
- Genomic DNA analysis: Staphylococcus aureus genomic DNA
- This enzyme shows equivalent activity at 37°C and 30°C, but stability of the enzyme in reaction mixtures is lower at 37°C than at 30°C.
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
SmaI restriction site:
CCC | GGG
GGG | CCC
Supplied buffer: T+BSA
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 30°C
- Source: Serratia marcescens Sb
- Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: lambda DNA
- Genomic DNA analysis: Staphylococcus aureus genomic DNA
- This enzyme shows equivalent activity at 37°C and 30°C, but stability of the enzyme in reaction mixtures is lower at 37°C than at 30°C.
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
