Retro-X Integration Site Analysis Kit
Retro-X Integration Site Analysis Kit
Brand: Takara Bio.
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Retro-X Integration Site Analysis Kit
The Retro-X Integration Site Analysis Kit contains all the reagents needed for PCR-based identification of retroviral vector integration sites in transduced cell clones. The first step is the construction of pools of adaptor-ligated genomic DNA fragments, referred to as "libraries". These libraries can then be used for identifying genomic DNA sequences flanking the 5' end of the integrated provirus using long-distance PCR.
Overview
- Simple determination of retrovirus integration sites using GenomeWalker technology
- Determine the precise site of integration of MMLV- and MSCV-based retroviruses
- Compatible with most commercially available retrovirus vectors
- Complete system including all the necessary enzymes, primers, controls, PCR kit, genomic DNA purification kit, and gel purification kit
- Lentiviral version also available for detecting integration sites for lentiviruses
Flow chart of the Retro-X integration site analysis protocol
Flow chart of the Retro-X integration site analysis protocol. The major steps include construction of three viral integration libraries, PCR amplification of genomic DNA from the libraries, and analysis of PCR products. The gel shows a typical result, the amplified bands can be purified and sequenced.
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Flow chart of the Retro-X integration site analysis protocol
Flow chart of the Retro-X integration site analysis protocol. The major steps include construction of three viral integration libraries, PCR amplification of genomic DNA from the libraries, and analysis of PCR products. The gel shows a typical result, the amplified bands can be purified and sequenced.
