NucleoSpin miRNA Plasma

Brand	MACHEREY-NAGEL

Applications

miRNA isolation from plasma / serum

Features

Processing of up to 900 μL sample volume possible
Optional on-column DNA digest for increased sensitivity in downstream applications
Simple and fast procedure without phenol / chloroform

Specifications

Technology: Silica membrane technology
Sample material: Plasma / serum (< 300 μL, < 900 μL with multiple loading steps)
Fragment size: ≥ 18 nt
Elution volume: 20–50 μL
Binding capacity: 200 μg
Processing time: 40 min/10 preps (without rDNase digestion), 70 min/10 preps (with rDNase digestion)

Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits

Get faster results with the NucleoSpin miRNA Plasma procedure than with competitor kits. MN (NucleoSpin miRNA Plasma): miRNA was isolated from 300 µl human blood plasma. Competitor Q: sample volumes of 1000 µl and 200 µl were used with kits for circulating nucleic acids (Q-CNA) and miRNA (Q-miRNA), respectively, according to competitor Q`s supplementary protocols. Competitor A: 300 µl plasma were processed according to the tissue protocol (6 volumes of lysis buffer) of a miRNA kit (A-miRNA).When each kit was used to perform 10 preps, the fastest results were obtained using NucleoSpin miRNA Plasma, both with and without rDNase digestion.