NucleoMag Pathogen
NucleoMag Pathogen
Brand: MACHEREY-NAGEL
In stock
SKU
NucleoMag Pathogen
96 sample magnetic bead-isolation of viral RNA and DNA and bacterial DNA from cell-free body fluids.
Applications
- Extraction of viral RNA and DNA and microbial DNA from clinical samples
- Clinical testing applications
Features
- One kit for any common clinical sample type
- High sensitivity
- Reliable nucleic acid isolation - suitable even for low viral titers
Specification
- Technology: Magnetic-bead technology
- Format: Highly reactive superparamagnetic beads
- Processing: Manual or automated
- Sample material: The prep is fully scalable. A convenient volume, especially for 96-well processing would be: < 200 µL whole blood, serum, plasma, < 25 mg tissue (e.g., ear notches), < 200 µL feces, < 200 µL swab wash solution
- Maximum amount of starting material in purification procedure: 200 µL liquid/homogenized sample
- Fragment size: 300 bp–approx. 50 kbp
- Elution volume: 50–100 µL
- Preparation time: 40–120 min/96 preps
- Binding capacity: 0.4 μg/μL beads
Meeting the requirements of the molecular diagnostic market
The NucleoMag® Pathogen kit allows rapid manual and automated small scale purification of viral RNA /DNA and microbial DNA using magnetic bead technology. The kit* is designed for processing of common clinical sample material, such as whole blood, serum or plasma, feces, tissue, or swabs. After sample specific pretreatment, liquid or homogenized sample is used in the purification procedure. The NucleoMag® Pathogen kit already contains carrier RNA, proteinase K and all buffers are delivered ready to use.
Sensitivity screening for Hepatitis B virus (HBV) and Hepatitis C virus (HCV)
Dilution (1:10) series of human plasma (200 µL, with original virus titer as shown) were extracted in triplicate with the NucleoMag® Pathogen kit and tested with RealStar® HBV PCR Kit 1.0 and the HCV RT-PCR Kit 1.0 (altona diagnostics). The NucleoMag® Pathogen kit demonstrates a sufficient sensitivity for Hepatitis B virus (HBV) and Hepatitis C virus (HCV) detection in human plasma sample material. The internal control was extracted and recovered as expected. PCR inhibitors were not detected.
Sensitivity screening for pathogen detection in human feces samples
Dilution series of 10–3–10–4 of feces samples for sensitivity screening was conducted using three (3) replicates. Extraction was performed with the NucleoMag® Pathogen kit and the competitor kit “R”. PCR analysis was performed using the RIDA® GENE Viral Stool Panel I (R-Biopharm) and RealStar® Clostridium difficile PCR Kit 1.0 (altona diagnostics).
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Meeting the requirements of the molecular diagnostic market
The NucleoMag® Pathogen kit allows rapid manual and automated small scale purification of viral RNA /DNA and microbial DNA using magnetic bead technology. The kit* is designed for processing of common clinical sample material, such as whole blood, serum or plasma, feces, tissue, or swabs. After sample specific pretreatment, liquid or homogenized sample is used in the purification procedure. The NucleoMag® Pathogen kit already contains carrier RNA, proteinase K and all buffers are delivered ready to use.
Sensitivity screening for Hepatitis B virus (HBV) and Hepatitis C virus (HCV)
Dilution (1:10) series of human plasma (200 µL, with original virus titer as shown) were extracted in triplicate with the NucleoMag® Pathogen kit and tested with RealStar® HBV PCR Kit 1.0 and the HCV RT-PCR Kit 1.0 (altona diagnostics). The NucleoMag® Pathogen kit demonstrates a sufficient sensitivity for Hepatitis B virus (HBV) and Hepatitis C virus (HCV) detection in human plasma sample material. The internal control was extracted and recovered as expected. PCR inhibitors were not detected.
Sensitivity screening for pathogen detection in human feces samples
Dilution series of 10–3–10–4 of feces samples for sensitivity screening was conducted using three (3) replicates. Extraction was performed with the NucleoMag® Pathogen kit and the competitor kit “R”. PCR analysis was performed using the RIDA® GENE Viral Stool Panel I (R-Biopharm) and RealStar® Clostridium difficile PCR Kit 1.0 (altona diagnostics).
