The Lenti-X Tet-One Inducible Expression System is a tetracycline-inducible lentiviral gene expression system that allows you to produce high titers of recombinant, VSV-G-pseudotyped lentiviruses for the purpose of establishing a tightly inducible expression system for your gene of interest in a wide variety of dividing and non-dividing mammalian cells. The all-in-one pLVX-TetOne Vector expresses the Tet-On 3G transactivator from the constitutive human PGK promoter in the forward orientation and your gene of interest from the PTRE3GS promoter in the reverse orientation. In the presence of doxycycline (Dox), the Tet-On 3G transactivator specifically binds and activates high-level transcription from the inducible promoter that controls expression of your gene. This vector does not contain a selection marker. The kit also includes Lenti-X Packaging Single Shots (VSV-G), a complete fourth generation lentiviral packaging system, and a sample of Lenti-X GoStix.
Overview
- Highly efficient Lenti-X Packaging Single Shots (VSV-G), (included) generates high titers with a very high safety profile
- Tightly controlled expression of your gene of interest with the TRE3G promoter
- Simple, single-vector design
- Option to use a puromycin resistance cassette to select for stable clones
- Tet-On 3G lentiviral systems are also available
- Tetracycline-inducible expression in the widest range of hard-to-transfect cell types
