AAV real-time PCR titration kit

The AAVpro Titration Kit (for Real Time PCR) is a kit for determining the titer of adeno-associated virus (AAV) using real-time PCR. This kit contains all of the reagents necessary for extraction of AAV particles from AAV vector-producing cells and real-time PCR assay of virus genome. This kit allows more precise and rapid quantification than conventional DNA blotting or ELISA methods. Quantification is based on amplification of the ITRs (inverted terminal repeats) of AAV, and therefore titer measurement is possible for various serotypes of AAV.

Overview

Can be used to determine the titer of AAV of any serotype as long as the ITRs are from AAV2 (most commonly used constructs)
Kit contains all reagents necessary for extraction of AAV particles from virus-producing cells and real-time PCR assay of AAV2 ITRs
Simple and fast titration method—results in approximately 2.5 hours
High-sensitivity titration—more precise quantification than conventional DNA blot and ELISA methods

Applications

Determining the titer of AAV using real-time PCR
Rapid and precise titration of any AAV serotype; quantification is based on the ITRs of AAV2, a sequence that is present in commonly used AAV vectors

Titer measurement of AAV by real-time PCR using ITR and CMV promoter targets

Titer measurement of AAV by real-time PCR using ITR and CMV promoter targets. The viral genomic titers of AAV1, 2, and 6 particles were evaluated using two primer sets, one for the CMV region and one for the ITR region (AAVpro titration kit). For each serotype, there was no difference in the viral titer determined by real-time PCR amplification of either the CMV or ITR region. This data indicates that the AAVpro Titration kit can be used for titration of any serotypes that have genomic AAV2 ITRs.