LA Taq DNA Polymerase with GC buffers
TaKaRa LA Taq DNA Polymerase with GC Buffer has the TaKaRa LA Taq DNA Polymerase blend for long-and-accurate PCR paired with GC-optimized buffers to power through difficult-to-amplify GC-rich sequences, including genomic DNA. The GC-optimized Buffers I and II are specifically designed to amplify GC-rich DNA templates (up to 73% GC content) or templates with a significant amount of secondary structure:
- GC Buffer I is recommended for amplification of fragments that are ≥5 kb in length
- GC Buffer II is recommended for fragments that are 2–3 kb in length
For the longest amplicons that are also GC rich, try PrimeSTAR GXL DNA Polymerase, which is designed to amplify genomic DNA up to 30 kb.
Overview
- Long and accurate PCR amplification of genomic DNA targets (long-range PCR)
- Optimized buffers (GC Buffers I & II) for amplification of templates with high GC content and/or significant secondary structure
- High-yield amplification of long DNA targets with minimal optimization
PCR products
PCR products generated with Takara LA Taq contain a mixture of 3′-A overhangs and blunt ends, which allows >80% cloning efficiency into T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low. It is also possible to clone the product in blunt-end vectors after blunting and phosphorylation of the end.
