Xfect mESC Transfection Reagent

Effective transfection of embryonic stem cells with Xfect mESC Transfection Reagent

Effective transfection of embryonic stem cells with Xfect mESC Transfection Reagent. ES-E14TG2a mES cells were transfected with 5 µg of DsRed-Express2 plasmid using 250 µg of Xfect mESC Transfection Reagent. 48 hr posttransfection, transfection efficiency was assessed via flow cytometry (left panel) as well as phase and fluorescent microscopy (middle and right panels, respectively). As quantified by flow cytometric analysis, 93.9 of the cells transfected with Xfect mESC Transfection Reagent were positive for DsRed-Express2 expression (left panel), with an MFI of 716.9 (data not shown).

Xfect mESC Transfection Reagent provides superior transfection efficiency in mES cells

Xfect mESC Transfection Reagent provides superior transfection efficiency in mES cells. Xfect mESC Transfection Reagent and three competitor reagents were used according to their respective protocols to transfect mES cell lines using plasmid DNA encoding AcGFP1, in a 6-well format. 48 hr posttransfection, AcGFP1 expression was assessed by flow cytometry in order to determine transfection efficiency.

Xfect mESC Transfection Reagent exhibits far higher transfection efficiency than leading competitor reagents

Xfect mESC Transfection Reagent exhibits far higher transfection efficiency than leading competitor reagents. ES-E14TG2a mES cells were transfected in a 6-well plate format with a plasmid expressing AcGFP1 using Xfect mESC Transfection Reagent (Panel A), Product L (Panel B), Product LX (Panel C), or Product F (Panel D) according to each manufacturer’s recommended protocol. 48 hr posttransfection, the cells were imaged using white light (top) and by fluorescence microscopy using a Zeiss Axioskop microscope equipped with a GFP filter (bottom).