The recent emergence of the coronavirus SARS-CoV-2 (COVID-19) has led to a need for rapid and reliable detection. Takara Bio offers solutions to support researchers and clinicians studying this novel coronavirus, such as viral RNA purification kits and one-step RT-qPCR mixes that can be used with established primers for sensitive detection of COVID-19 down to five viral copies.
Reliable detection of COVID-19 at 5, 50, 500, 5,000, and 50,000 copies with our one-step RT-qPCR kits. These data were generated by Takara Bio using publicly available protocols from the CDC. These data have not been validated by the CDC.
Explore industry-leading products that can advance your COVID-19 research:
NucleoMag® PathogenThe NucleoMag Pathogen kit is designed for the isolation of viral RNA and DNA and bacterial DNA from cell-free body fluids such as serum or plasma, blood, homogenized tissue sample suspensions, stool sample suspensions, and swab washes. This kit provides reagents and magnetic beads for isolation of 96 samples.
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NucleoSpin RNA VirusNucleoSpin RNA Virus is designed for the isolation of viral nucleic acids from serum, plasma, or any cell-free biological fluid.
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One Step PrimeScript™ III RT-PCR KitOne Step PrimeScript III RT-qPCR Mix is a dedicated reagent for one-step real-time, probe-based RT-qPCR (using the 5’ nuclease method). This 2X premix does not freeze at its storage temperature of -20℃, so a reaction can be started simply by adding the template sample, primer, and a probe for detecting the desired target. The quick and simple protocol allows the reverse transcription and qPCR reactions to be performed in the same tube. The reverse transcription reaction uses the novel PrimeScript III RTase, which displays increased heat tolerance (up to 55℃) while maintaining the specificity and extensibility of PrimeScript RTase. This allows cDNA synthesis from RNA with a more complex secondary structure. After cDNA synthesis, TaKaRa Taq HS performs highly specific and efficient PCR amplification, while the fluorescence emitted by the probe is detected in real-time. One Step PrimeScript III RT-qPCR Mix is also highly resistant to a wide variety of inhibitory substances such as heparin (blood) and humic acid (soil), allowing stable one-step real-time RT-qPCR to be performed on a wide range of samples. This product can be used for various applications such as gene expression, RNA virus detection, etc.
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One Step PrimeScript™ RT-PCR Kit (Perfect Real Time)The One Step PrimeScript RT-PCR Kit (Perfect Real Time) is designed for one step, real-time reverse transcription PCR (RT-PCR) using probe detection. With this kit, all RT-PCR steps can be performed in a single tube. Therefore, it is not necessary to add additional reagents during the reaction, minimizing the risk of contamination and simplifying the workflow. Amplified products are monitored in real time without need for gel electrophoresis after PCR. This kit is suitable for detection of small amounts of RNA. This kit uses PrimeScript Reverse Transcriptase, which allows efficient and rapid cDNA synthesis, and Takara Ex Taq HS, which enables highly efficient hot start PCR. |
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PrimeDirect™ Probe RT-qPCR MixPrimeDirect Probe RT-qPCR Mix is designed for one-step, real-time RT-PCR via probe detection (5′-nuclease method). This product is supplied as a 2X premix to facilitate the easy preparation of reaction mixtures. It requires only the addition of your primer, probe, and sample, and the RT-qPCR can be performed by simply adding your sample directly to the reaction mixture without intervening nucleic acid purification steps. Because this product is highly resistant to various PCR inhibitors such as heparin (blood) and humic acid (soil), it can be used to directly detect pathogens, including viruses and bacteria, in various biological specimens and to directly analyze gene expression in cells.
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