AAVpro Extraction Solution
Isolation of AAV particles from AAV particle-producing cells is conventionally performed using the freeze-thaw or sonication methods. However, these methods are time consuming and require special equipment. The AAVpro Extraction Solution is a reagent for the extraction of AAV particles from AAV particle-producing cells. This reagent provides a simple and efficient method for AAV particle isolation; the reagents are added to virus-producing cells and viral particles are recovered by centrifugation. The extracted AAV particle solution contains only a small amount of host protein and nucleic acid contamination. The resulting viral extract is well-suited for cell infection or further purification of viral particles.
Overview
- Three-fold increase in viral yield compared to feeeze-thaw methods
- Simple method for AAV particle extraction from virus-producing cells
- Reduced host DNA and protein contamination
- Can be used for any AAV serotype
Applications
- AAV particle extraction from virus-producing cells
