ThruPLEX DNA-Seq Kit: all-purpose DNA library preparation for Illumina NGS platforms
The ThruPLEX DNA-Seq Kit builds on innovative ThruPLEX chemistry to generate DNA libraries from as little as 50 pg of DNA, and improves sensitivity while providing up to 384 indexes for multiplexing. This kit can be used with fragmented double-stranded DNA (dsDNA) from any sample source—genomic DNA, biofluids such as cell-free DNA (cfDNA), DNA from FFPE materials, and cDNA.
The ThruPLEX DNA-Seq Kit has been reconfigured from the original versions of ThruPLEX kits and does not include indexes. Compatible Index kits are described below and can be purchased.
The entire ThruPLEX DNA-Seq Kit workflow is performed in a single tube or well in about two hours and requires no purification steps or sample transfers. The kit can be used in any DNA-seq application, RNA-seq, or ChIP-seq and offers robust target enrichment performance with all of the leading platforms.
Overview
- High performance from low inputs—generates libraries from 50 pg to 50 ng of input material
- Fast and simple workflow—three-step, single-tube or single-well protocol takes only about two hours, no transfers necessary
- Compatible with major target enrichment platforms—suitable for use with Agilent SureSelect, Roche Nimblegen SeqCap EZ, and IDT xGen Lockdown probes
