Van91I (PflMI)

Brand	Takara Bio

Van91I (PflMI) restriction site:

CCAN NNN | NTGG
GGTN | NNN NACC

Supplied buffer: K

Effect of DNA methylation: enzyme activity is affected by dcm methylase

Reaction temperature: 37°C

Source: Vibrio anguillarum RFL 91
Concentration: 4–12 units/µl
Substrate for unit definition: lambda DNA
Star activity: unrelated site may often be cut in the presence of a high concentration of glycerol
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning