PrimeSTAR GXL DNA Polymerase
PrimeSTAR GXL DNA Polymerase provides efficient PCR amplification even for the toughest scenarios, including amplification of GC-rich targets, amplification in the presence of excess template that can contribute to nonspecific binding, and amplification of long products (up to 30 kb). The antibody-mediated hot-start feature enhances PCR specificity and sensitivity.
PrimeSTAR GXL polymerase comes with separate tubes of 5X buffer and dNTP mixture.PrimeSTAR GXL polymerase is our most robust high-fidelity polymerase available for challenging targets (GC-rich sequences, excess template, long amplicons).
Overview
- Amplification of products up to 30 kb (human genomic DNA), 40 kb (lambda DNA), or 13.5 kb (human cDNA)
- Antibody-mediated hot-start formulation
- High tolerance for GC-rich templates as well as the presence of excess nucleic acids templates
Applications
- Reliable amplification of long, GC-rich, or AT-rich sequences, such as regulatory regions and repeats
- HLA typing
- Unbiased library amplification for NGS
Termini of PCR products
- PrimeSTAR GXL DNA Polymerase generates products with blunt ends. It is suitable for use in conjunction with blunt-ended cloning systems.
Recommended maximum amplicon length
- Up to 30 kb (human genomic DNA template)
- Up to 40 kb (lambda DNA template)
- Up to 13.5 kb (human cDNA template)
