pmR-mCherry Vector

Additional information

Brand

Size

20 ug

pmR-mCherry is a mammalian expression vector designed to constitutively coexpress a microRNA (miRNA) of interest and the mCherry fluorescent protein. mCherry is a mutant fluorescent protein derived from the bright red fluorescent protein, DsRed. The unmodified vector can be used to express mCherry in mammalian cells.

Overview

  • Strong coexpression of miRNA and a bright fluorescent protein
  • Clone your miRNA into the 3′ UTR of mCherry or ZsGreen1
  • Simultaneously verify miRNA expression and transfection efficiency via fluorescent microscopy

Applications

  • Over-expression of microRNAs to study gene or microRNA function

The pmR-mCherry and pmR-ZsGreen1 vectors will coexpress a fluorescent protein and a miRNA sequence that is embedded in the 3′ UTR of the vector’s mRNA transcript

The pmR-mCherry and pmR-ZsGreen1 vectors will coexpress a fluorescent protein and a miRNA sequence that is embedded in the 3' UTR of the vector’s mRNA transcript

The pmR-mCherry and pmR-ZsGreen1 vectors will coexpress a fluorescent protein and a miRNA sequence that is embedded in the 3′ UTR of the vector’s mRNA transcript. miRNA expression can be selected for and/or verified in transfected cells by monitoring red or green fluorescence.

The pmR-mCherry and pmR-ZsGreen1 Vectors

The pmR-mCherry and pmR-ZsGreen1 Vectors

The pmR-mCherry and pmR-ZsGreen1 Vectors. Map of the vectors (Panel A). Cells transfected with the vectors express your miRNA and exhibit red or green fluorescence (Panel B).