pmCherry-N1 is a mammalian expression vector designed to express a protein of interest fused to the N-terminus of mCherry, a mutant fluorescent protein derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed. The unmodified vector can be used to express mCherry in mammalian cells.
Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
mCherry-fusion constructs. HeLa cells were transiently transfected, via a lipid-based method, with mammalian expression vectors encoding mCherry fused to either human cytoplasmic actin (Panel A) or tubulin (Panel B). Cells were fixed using 4 paraformaldehyde and imaged 36 hr posttransfection with a 40X objective on a Zeiss Axioskop microscope using the 575/50, 610, 640/50 filter set.
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