Applications
- Isolation of RNA from fresh or frozen whole blood from common blood collection tubes (EDTA, citrate, heparin)
- Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology
Features
- Direct total blood lysis – very simple and convenient procedure (patent pending)
- No selective erythrocyte lysis at 4 °C – complete processing at room temperature
- Superior RNA yield and quality from up to 1.3 mL whole blood
- Efficient on-column DNA removal for an increased sensitivity in downstream applications
- Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin)
Specifications
- Technology: Silica membrane technology
- A260/A280: 1.9–2.1
- Fragment size: > 200 nt
- Processing: Centrifugation
- Sample material: 400–1300 μL blood
- Typical yield: Blood (1300 μL): 4–26 μg*
- Elution volume: 200–400 μL
- Binding capacity: 700 μg
- Processing time: 75 min/6 preps
