Applications
- Clean-up and concentration of pre-purified DNA (organic extractions)
- Clean-up and concentration of DNA from enzymatic reactions
- Not recommended for gel or PCR clean-up! For this application, please use NucleoSpin® Gel and PCR Clean-up.
- Typical downstream applications: qPCR, enzymatic reactions, Southern blotting, STR amplification
Features
- Effective post clean-up and concentration of large DNA fragments for successful downstream applications
- High recovery rates up to 90 % for DNA fragments 100 bp–50 kbp
- Concentrate your DNA sample easily and faster compared to microdialysis filtrations units
- 15 min/10 preps – time-saving procedure
Specifications
- Technology: Silica membrane technology
- Fragment size: 100 bp–approx. 50 kbp
- A260/A280: 1.8–1.9
- Sample material: Aqueous DNA solution (< 150 μL incl. < 25 μg DNA)
- Typical recovery: 80–90 %
- Elution volume: 50–100 μL
- Binding capacity: 50 μg
- Processing time: 10 min/6 preps
