Applications
- Manual and automated isolation of circulating cell-free DNA from human plasma, derived from e.g., EDTA blood draw tubes or Cell-Free DNA BCT® for “Liquid Biopsy” analysis
- Typical downstream applications: NGS, qPCR, ddPCR
Features
- Consistent cfDNA recovery from 1–10 mL plasma samples
- Efficient purification of fragmented DNA as small as 50 bp
- No PCR inhibition regardless of your preferred sample volume
Specifications
- Technology: Magnetic-bead technology
- Format: Highly reactive superparamagnetic beads
- Processing: Manual or automated
- Sample material: 1–10 mL human EDTA/Cell-Free DNA BCT® plasma
- Fragment size: > 50 bp
- Typical yield: Depending on sample source, storage, and quality
- A260/A280: 1.6–1.9
- Elution volume: 50–200 µL
- Preparation time: 60 min/24 preps (2 mL; excl. lysis)*
- Binding capacity: 0.3 µg/µL beads
