MazF is a toxin protein in the toxin-antitoxin module of E. coli. It possesses endoribonuclease activity and specifically cleaves single-stranded RNA at the 5′ end of ACA sequences. This enzyme does not cleave double-stranded RNA, double-stranded DNA or single-stranded DNA. This product, mRNA Interferase-MazF, is supplied as a fusion protein of E. coli MazF and Trigger Factor (TF) an E. coli chaperone protein. The enzyme is also supplied with a 5X MazF buffer (200mM Sodium phosphate, pH 7.5, 0.05% Tween-20).
Applications
- Use for site-dependent cleavage of ssRNA
Source
Expressed as a recombinant protein in Escherichia coli.
