HAT-tag Expression System

FPLC purification of HAT-tagged protein with TALON Superflow

FPLC purification of HAT-tagged protein with TALON Superflow. E. coli cells were extracted in 50 mM sodium phosphate, 0.3 M NaCl, 5 mM imidazole, pH 7.0 and eluted in 150 mM imidazole (1). Panel A. Cell lysate was purified with a TALON Superflow Column. Panel B. SDS-PAGE analysis of the procedure. M: molecular weight marker.

pHAT10/11/12 and pHAT20 Vector maps

pHAT10/11/12 and pHAT20 Vector maps.

Amino acid sequences of 6xHis, 6xHN, and HAT polyhistidine tags

Amino acid sequences of 6xHis, 6xHN, and HAT polyhistidine tags.

Features and benefits of the HAT (histidine affinity tag) Protein Expression & Purification System, which allows proteins expressed in bacteria to be purified at neutral or physiological pH and in native or denaturing conditions

Features and benefits of the HAT (histidine affinity tag) Protein Expression & Purification System, which allows proteins expressed in bacteria to be purified at neutral or physiological pH and in native or denaturing conditions.