- Streamlined workflow with high reproducibility
- Robust performance with FFPE and cfDNA
- Accurate detection of rare mutations with molecular tags
- Coverage for a broad range of GC content with microbiome samples
- Unique dual indexes for use on the NovaSeq™ system
Simple workflow: one tube and done
Addition-only steps provide a faster, failproof library prep experience without lengthy bead purifications that cause sample loss and impact data quality. Without hidden steps—like adapter dilution—it is easy to automate, scale, and standardize. Pre-plated, single-use UDI indexes make sample assignment clear for your precious materials. Learn more
Figure 1. ThruPLEX HV chemistry features a fast, simple workflow without bead purification after ligation—saving time, money, and resources
Reproducible high-quality data from FFPE
Formalin-fixed paraffin-embedded samples are a gold mine of data for understanding disease—if you are equipped to tackle these challenging samples. ThruPLEX HV kits have been optimized to produce high-quality, reproducible data from FFPE tissue. View data »
Figure 2. Expect highly reproducible and consistent performance at a broad range of inputs, even for highly degraded FFPE DNA.
Reliably detect low-frequency mutations from cfDNA with molecular tags
Cell-free samples, such as plasma, pose significant challenges to scientists interested in using minimally invasive techniques to look at DNA—especially when the biomarkers of interest are rare. ThruPLEX Tag-Seq HV provides an elegant solution, with chemistry designed to generate complex libraries and molecular tags that enable accurate detection of rare mutations. View data
Figure 3. Accurately detect low-frequency mutations with the ThruPLEX Tag-Seq HV system, featuring discrete, balanced molecular tags.
