Advantage GC Genomic LA Polymerase Mix
Advantage Genomic LA Polymerase Mix and Advantage GC Genomic LA Polymerase Mix are designed for long and accurate (LA) PCR amplification. These mixes offer 6.5X higher fidelity than wild-type Taq DNA polymerase, and allow synthesis of products up to 30 kb for complex templates such as genomic DNA, and up to 48 kb for less complex templates such as λ DNA. Both versions of Advantage Genomic GC Polymerase Mix are composed of a full-length Taq DNA polymerase, a small amount of proofreading enzyme, and a hot-start antibody, and are supplied with optimized buffers.
The Advantage GC Genomic LA Polymerase Mix includes a 2X Advantage GC-Melt buffer to allow efficient amplification of GC-rich templates, which have strong secondary structures that resist denaturation and prevent efficient primer annealing. This kit can handle GC-rich sequences (up to 90% GC) that resist standard PCR amplification techniques, and enables synthesis of PCR products up to 20 kb from GC-rich human genomic DNA.
Overview
- Designed for ‘long and accurate’ PCR amplification—amplify products as large as 48 kb
- 6.5X higher fidelity than wild-type Taq DNA polymerase—generates virtually error-free PCR products
- High sensitivity—amplify regions of GC-rich DNA, such as a 1.9-kb region of the TGF-beta gene (GC content = 69%), from as little as 10 pg of template
- Antibody-mediated hot start—increase specificity by preventing nonspecific amplification before thermal cycling
- Advantage GC-Melt Buffer system weakens base pairing in GC-rich sequences—optimized for use with complex, GC-rich genomic templates
Applications
- Long PCR
- GC-rich PCR
- Difficult PCR
