Complete, sensitive, and fast viral genomics with SMARTer Stranded Kit

Complete, sensitive and fast viral genomics with SMARTer® Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian

Sequencing the SARS-CoV-2 virus causing COVID-19 pandemics is crucial for public health authorities to understand identity and genomic changes of the virus and its transmission patterns.

Takara Bio supports researchers and clinicians studying this novel coronavirus with the SMARTer® Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian, which was used for SARS-CoV-2 virus sequencing.

Widely used solution for viral genomics

Thanks to built-in human ribosomal depletion and fast protocol, the SMARTer® Stranded kit has been widely used by world leading institutes for viral genomics and genomic epidemiology.

Virus Sample Type Reference
SARS-CoV-2 Bronchoalveolar lavage fluid Wu, F. et al., A new coronavirus associated with human respiratory disease in China. Nature. Mar;579(7798):265-269. (2020)
Metatranscriptomics – infectome Throat swabs Li CX. et al., High resolution metagenomic characterization of complex infectomes in paediatric acute respiratory infection. Sci Rep. Mar 3;10(1):3963. (2020)
HIV Plasma Bonsall D. et al., A comprehensive genomics solution for HIV surveillance and clinical monitoring in a global health setting. bioRxiv 2018.08.28.397083 (2018)
TBEV (Tick-borne encephalitis virus) Infected cells Cheval J. et al., Adventitious Virus Detection in Cells by High-Throughput Sequencing of Newly Synthesized RNAs: Unambiguous Differentiation of Cell Infection from Carryover of Viral Nucleic Acids. mSphere. Jun 5;4(3). pii: e00298-19. (2019)
MRV (Mammalian orthoreovirus) Infected cells Yamamoto S.P. et al., Novel human reovirus isolated from children and its long-term circulation with reassortments. Sci Rep. Jan 22;10(1):963. (2020)
Jingmenvirus Serum Temmam S. et al., Insights into the Host Range, Genetic Diversity, and Geographical Distribution of Jingmenviruses. mSphere. Nov 6;4(6). pii: e00645-19. (2019)
HBV (Hepatitis B virus) CSF (cerebrospinal fluid) Pettersson J.H. et al., Meta-transcriptomic identification of hepatitis B virus in cerebrospinal fluid in patients with central nervous system disease. Diagn Microbiol Infect Dis. Dec;95(4):114878. (2019)
HHV-4 (Epstein-Barr) Infected cells Brussel A. et al., Use of a new RNA next generation sequencing approach for the specific detection of virus infection in cells. Biologicals. May;59:29-36. (2019)

Explore industry-leading products that can advance your COVID-19 research:

SMARTer® Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian

The SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian is used to generate strand-specific RNA-seq libraries for Illumina sequencing from 250 pg–10 ng inputs of purified total RNA. This kit incorporates Takara Bio’s proprietary SMART (Switching Mechanism at the 5’ end of RNA Template) technology and includes refinements to the SMARTer method for stranded RNA-seq that simplify the library preparation workflow and improve sequencing performance. This method was developed to work with either high- or low-quality total RNA, does not require additional rRNA removal methods or kits, and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNA—typically present in high abundance following cDNA synthesis from total RNA inputs—makes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA.

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SMART-Seq® Stranded Kit

The SMART-Seq Stranded Kit is used to generate strand-specific RNA-seq libraries for Illumina® sequencing from 1–1,000 sorted cells or 10 pg–10 ng of purified total RNA. This kit incorporates Takara Bio’s SMART (Switching Mechanism at the 5′ end of RNA Template) technology and includes refinements to the SMARTer method for stranded RNA-seq that simplify the library preparation workflow and improve sequencing performance.

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