EmeraldAmp MAX HS PCR Master Mix
EmeraldAmp MAX HS PCR Master Mix is the antibody-mediated hot-start (HS) version of EmeraldAmp MAX PCR Master Mix. The inclusion of an antibody prevents mispriming and non-specific amplification during PCR setup.
EmeraldAmp Max HS PCR Master Mix can be used for routine PCR applications, and completed reactions can be analyzed directly via gel electrophoresis. This hot-start master mix includes a high-yield HS PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (green), and a density reagent in a 2X Taq PCR master mix format. Only primers and DNA template need to be added to initiate the reaction.
Overview
- Antibody-mediated hot start—minimizes nonspecific amplification and primer dimers
- High yield—at least 10 times more end product than conventional PCR
- Excellent performance—optimized buffer for better performance with AT- or GC-rich targets
- Convenience—simply add template, primers, and water to the 2X Taq PCR Master Mix, then load the reactions directly onto a gel upon completion of PCR
- Emerald green loading dye—easy tracking during electrophoresis
- Easy integration with downstream applications—restriction enzyme digests can be performed directly in the PCR buffer
Applications
- TA cloning or sequencing
- Direct gel electrophoresis
- Long and high-yield PCR
