Applications
RNA clean-up of
- Pre-purified RNA (e.g., with TRIzol®)
- DNase digestions (e.g., with MACHEREY-NAGEL rDNase Set)
- Reaction mixtures
- Up to 55-fold increase in concentration
- Typical downstream applications: enzymatic labeling reactions, RT-PCR, DNA/RNA-based chip hybridization
Features
- Complete removal of RT-PCR inhibitors
- Very high RNA recovery and concentration
- RNA clean-up from pre-purified RNA (phenol / chloroform), enzymatic reactions (e.g., amplification reactions, labeling reactions, DNase digestions)
- Time-saving procedure based on NucleoSpin® RNA XS technology
- Input volume up to 300 μL
- Elution in as little as 5 μL
Specifications
- Technology: Silica membrane technology
- Fragment size: > 200 bp
- A260/A280: 1.9–2.1
- Sample material: RNA solution (< 300 μL incl. < 90 μg RNA)
- Typical recovery: 85–95 %
- Elution volume: 5–30 μL
- Binding capacity: 110 μg
- Processing time: 20 min/6 preps
