Applications
- Purification of PCR products
- Extraction of DNA / RNA from agarose and polyacrylamide gels
- Typical downstream applications: clining, sequencing, PCR, restriction analysis
- NucleoSpin gel and PCR Clean-up ensures complete removal of contaminations auch as:
- nucleoties, primers
- enzymes
- mineral oil
- PCR additives (e.g., saltes betaine, DMSO)
- detergents (e.g., Tween 20, Triton X-100)
- dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti® Safe GelStain, DNA SafeStain)
Features
- Two applications in one kit – one buffer with optimal performance for both applications
- High recoveries for fragments down to 50 bp
- Binding buffer with pH indicator
- Separate buffers for single stranded DNA or SDS containing samples available
- Suitable for all gel buffer systems (e.g., TAE, TBE)
Specifications
- Technology: Silica membrane technology
- Processing: Centrifugation
- Sample material: < 10 mL PCR reaction mixture, < 10 g TAE/TBE agarose gel
- Fragment size: 50 bp–approx. 20 kbp
- Typical recovery: 70–95 %
- A260/A280: 1.75–1.85
- Elution volume: 1000 µL
- Binding capacity: 250 µg
- Processing time: 30 min/6 preps
