VpaK11BI (AvaII)

Brand	Takara Bio

VpaK11BI (AvaII) restriction site:

G | G(A/T)C C
C C(T/A)G | G

Supplied buffer: basal

Effect of DNA methylation: enzyme activity is affected by both dcm and CG methylase

Reaction temperature: 30°C

Source: Vibrio parahaemolyticus 1743-1
Concentration: 4–12 units/µl
Substrate for unit definition: lambda DNA
Star activity: cleavage at alternative site(s) occurs in the presence of high concentrations of glycerol, DMSO, and at alkaline pH or low ionic strength.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning