Linear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 poly-adenylation signal. The 1.8-kb marker is compatible with any expression vector (including Tet System vectors) for generating stable transfectants using CalPhos transfection reagent or a liposome-mediated transfection protocol.
Overview
- Both the linear selection markers and the pTK-Hyg Vector and pPur Vector may be used in cotransfections to establish stable cell lines
- Linear selection markers require you to screen fewer clones, resulting in more positive clones than when circular plasmid markers are used
- Linear selection markers are ideal for use with all Takara Bio’s Tet-On and Tet-Off Inducible Expression Systems—screen fewer candidates and find more highly inducible clones
