NucleoMag Pathogen

Meeting the requirements of the molecular diagnostic market

The NucleoMag® Pathogen kit allows rapid manual and automated small scale purification of viral RNA /DNA and microbial DNA using magnetic bead technology. The kit* is designed for processing of common clinical sample material, such as whole blood, serum or plasma, feces, tissue, or swabs. After sample specific pretreatment, liquid or homogenized sample is used in the purification procedure. The NucleoMag® Pathogen kit already contains carrier RNA, proteinase K and all buffers are delivered ready to use.

Sensitivity screening for Hepatitis B virus (HBV) and Hepatitis C virus (HCV)

Dilution (1:10) series of human plasma (200 µL, with original virus titer as shown) were extracted in triplicate with the NucleoMag® Pathogen kit and tested with RealStar® HBV PCR Kit 1.0 and the HCV RT-PCR Kit 1.0 (altona diagnostics). The NucleoMag® Pathogen kit demonstrates a sufficient sensitivity for Hepatitis B virus (HBV) and Hepatitis C virus (HCV) detection in human plasma sample material. The internal control was extracted and recovered as expected. PCR inhibitors were not detected.

Sensitivity screening for pathogen detection in human feces samples

Dilution series of 10–3–10–4 of feces samples for sensitivity screening was conducted using three (3) replicates. Extraction was performed with the NucleoMag® Pathogen kit and the competitor kit “R”. PCR analysis was performed using the RIDA® GENE Viral Stool Panel I (R-Biopharm) and RealStar® Clostridium difficile PCR Kit 1.0 (altona diagnostics).